HPLC法测定金嗓清音丸中丹皮酚和芍药苷的含量

目的建立HPLC法测定金嗓清音丸中丹皮酚和芍药苷的含量测定方法。方法采用HPLC法,以依利特Hypersil ODS2(5μm,4.6 mm×200 mm)为色谱柱,丹皮酚以甲醇-水(55∶45)为流动相,检测波长为274 nm。芍药苷以乙腈-0.033 mol·L-1磷酸二氢钾溶液(15∶85)为流动相,检测波长为230 nm。流速均为1.0 ml·min-1。结果丹皮酚的线性范围为0.0427～0.6835μg(r=0.9997),平均回收率为98.7%,RSD为0.73%(n=6);芍药苷的线性范围为0.2084～3.3344μg(r=0.9998),平均回收率为98.6%,RSD为0.96%(n=6)。结论此方法操作简便准确,重复性好,可用于该制剂的质量控制。

Determination of Paeonolum and Paeoniflorin in Jinsangqingyin Pills by HPLC

Objective To establish a method for determination of paeonolum and paeoniflorin in Jinsangqingyin pills by HPLC.Methods HPLC was applied on a Hypersil ODS2(5 μm,4.6 mm×200 mm) column.The mobile phase for paeonolum consisted of methanol and water(55∶ 45) at a detection wavelength of 274 nm.The flow rate was 1.0 ml · min-1.The mobile phase for paeoniflorin consisted of acetonitrile and 0.033 mol · L-1 potassium dihydrogen phosphate solution(15∶ 85) at a detection wavelength of 230 nm and a flow rate of 1 ml · min-1.Results The linear range of paeonolumand paeoniflorin was 0.0427-0.6835 μg(r=0.9997)and 0.2084-3.3344 μg(r=0.9998).The average recovery of paeonolumand paeoniflorin was 98.7% and 98.6%.RSD was 0.73% and 0.96%.Conclusion This method is simple,accurate,reproducible and suitable for quality control of this drug.