干细胞标志物Oct-4 Sox-2表达与结肠癌术后复发转移的关系

  目的  探讨结肠癌组织中Oct-4、Sox-2表达情况及其对术后复发转移的预测作用。  方法  采用免疫组织化学方法检测手术切除的80例结肠癌术后标本中Oct-4、Sox-2表达情况，并且对两指标的表达同肿瘤分化程度、分期和术后复发转移关系进行分析。采用RT-PCR检测20例冰冻肿瘤组织及癌旁组织中Sox-2、Oct-4表达情况。  结果  80例患者中35例出现复发转移，Sox-2、Oct-4在转移组中表达率分别为48.57%（17/35）、51.43%（18/35），在非转移组中表达率分别为17.78%（8/45）、13.33%（6/ 45），差异有统计学意义（P=0.001）。原发病灶分化程度、T分期和N分期同Oct-4和Sox-2表达无显著性差异。均为阳性表达者其转移发生率高，生存分析显示不同表达状态转移出现时间差异有统计学意义（P=0.001）。RT-PCR分析显示20例肿瘤组织中Sox-2、Oct-4阳性表达率分别为40%（8/20）、45%（9/20），明显高于癌旁正常组织5%（1/20）。  结论  肿瘤组织中Sox-2、Oct-4表达同结肠癌术后转移相关，两者联合检测有助于评估结肠癌术后复发转移的可能。 

Relationship of Oct-4 and Sox-2 Expression in Colon Carcinoma with Recurrence and Metastasis

  Objective  The current work aims to investigate the expression of Sox-2 and Oct-4 in colon carcinoma and its relationship with relapse and metastasis.  Methods  The expression of Oct-4 and Sox-2 was detected in 80 cases of colon cancer tissue by immunohistochemical assay. The correlation of the Oct-4 and Sox-2 expression with histological differentiation, T stage, N stage, and metastasis was analyzed. The level of Oct-4 and Sox-2 expression in the cancer and normal paraneoplastic tissues was evaluated using RT-PCR.  Results  Metastasis was observed in 35 of all the 80 cases studied. The positive rate of Sox-2 and Oct-4 in the metastasis group was 48.57% (17/35) and 51.43% (18/35), respectively, whereas it was 17.78% (8/45) and 13.33% (6/45) in the non-metastasis group. Significant differences were observed between the two groups (P = 0.001). The expression of Oct-4 and Sox-2 was not associated with differentiation, T stage, or N stage of the tumor. The group with positive expression of Oct-4 and Sox-2 showed high metastatic rate. The survival analysis showed that the time of metastasis was significantly different in the groups with various expressions (P = 0.001). The positive expression rate of Sox-2 and Oct-4 detected by RT-PCR in colonic carcinoma tissues was 40% (8/20) and 45% (9/20) respectively, higher than that in normal tissues.  Conclusion  The expression of Sox-2 and Oct-4 in tumor tissue can predict postoperative relapse and metastasis of colon carcinoma.