HPLC法测定蓝玉簪颗粒中三七皂苷R1、人参皂苷Rg1、Rb1的含量

目的：建立HPLC法测定蓝玉簪颗粒中三七皂苷R1与人参皂苷Rg1、Rb1含量的方法。方法：色谱柱为Agilent C18（250mm×416mm，5μm），以乙腈-水为流动相，梯度洗脱；检测波长为203nm；流速为1．0ml·min^-1；柱温为35℃。结果：三七皂苷R1在0．56—3．54峙、人参皂苷Rg1在0．41—8．12μg、人参皂苷Rb1在0．40～5．31μg范围内线性关系良好，相关系数r均为0．9999。三七皂苷R1与人参皂苷Rg1、Rb1的平均回收率分别是99．45％、99．11％、99．84％；RSD值分别为0．97％、0．56％、0．24％（n=6）。结论：本方法操作简便、可靠，重复性好，可作为蓝簪这颗粒中三七皂苷R1、人参皂苷Rg1、Rb1的含量测定方法。

Determination of Notnginsenoside R1,Ginsenoside Rg1,Rb1 in Lanyuzan Granules by HPLC

Objective： To develop a method for the determination of notoginsenoside R1 and ginsenoside Rg1, Rb1 in Lanyuzan granules by HPLC. Method： Agilent C18 column（250 mm ×416 mm, 5μm） was used, the mobile phase was acetanitrilewater with gradient elution, the detection wave length was at 203nm, the flow rate was 1.0 ml · min^-1 , and column temperature was 35℃. Result： A good linearity for notoginsenoside R1. ginsenoside Rg1 and ginsenoside Rb1 was within the range of 0.56 -3. 54μg（r = 0.999 9） ,0.41 -8.12μg（r =0.999 9） and 0.40 -5.31μg（r=0.999 9）, resepectivelv. Notoginsenoside R1 and ginsenoside Rg1,Rb1 , respectively, the average recovery is 99.45% , 99.11% , 99.84% ; RSD values were 0.97%, 0.56%, 0.24% （ n = 6）. Conclusion： The method is simple, accurate and with good reproducibility and can be used for the determination of Notoginsenoside R1 ginsenoside Rg1 , Rb1 in lanyuzan granules.