Caspase-3在脑出血后神经细胞凋亡中的作用

目的:探讨Caspase-3表达与大鼠脑出血后神经元凋亡的关系。方法:应用立体定向技术,将自体不凝血注入大鼠尾状核制备脑出血模型,将动物随机分为假手术组、脑出血组,分别在不同时间点断头取脑,连续切片作TUNEL染色和Caspase-3免疫组化染色。结果:脑出血后6h血肿内部及周边组织出现TUNEL阳性细胞, 72h达高峰, 2w时仍有少量表达;Caspase-3在脑出血后6h表达明显增高, 24h达到高峰,各组与假手术组之间差异显著(P<0 05 )。脑出血后的TUNEL阳性细胞与Caspase-3的表达呈正相关(r=0. 547,P<0 01),且Caspase-3的高峰时间早于凋亡的发生。结论:脑出血后的细胞凋亡与Caspase-3的表达有关。

Role of Caspase -3 in Neuronal Apoptosis After Experimental Intracerebral Hemorrhage in Rats

Objective To investigate the relationship between the expression of caspase-3 and neuronal apoptosis after experimental intracerebral hemorrhage (ICH) in rats. Methods ICH rat models were prepared with stereotactic infusiong of autologous blood into caudate nucleus. TUNEL method of staining was used to detect apoptotic cells and immunohistochemistry technic was applied to detect caspase-3 expression in cerebral tissue of the sacrificed animals at 6h,12h, 24h, 48h, 72h, 1w, 2w after the intracererbral blood infusion (n=5 in each group). Five rats underwent sham operation with intracerebral infusion of normal saline. Results At 6h, TONEL positive cells could be demonstrated with peak at 72h and could still be shown at 2w. Apoptotic cells were not seen in the sham-operation group. Caspase-3 expression was significant at 6h with peak at 24h and was significantly higher than those in the sham group (P<0.05). Expression of caspase-3 was positively correlated with the number of TUNEL positive cells (r=0.547, P<0.01). Conclusion The cellular (neuronal) apoptosis after experimental intracerebral hemorrhage was closely related to the expression of caspase-3, suggesting possible beneficial effect of selective caspase-3 inhibitors.