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| 高效液相色谱法测定胡芦巴中胡芦巴碱的含量 | |
| 引用本文： | 赵怀清,曲燕,王学娅,张洪建,李发美,服部征雄. 高效液相色谱法测定胡芦巴中胡芦巴碱的含量[J]. 中国中药杂志, 2002, 27(3): 194-196 |
| 作者姓名： | 赵怀清 曲燕 王学娅 张洪建 李发美 服部征雄 |
| 作者单位： | 1. 沈阳药科大学,药学院,辽宁,沈阳,110015 2. 辽宁省基础医学研究所,辽宁,沈阳,110005 3. 日本富山医科药科大学,日本，富山，930-0194 |
| 摘 要： | 目的 :建立高效液相色谱法测定胡芦巴中胡芦巴碱的方法。方法 :石油醚 无水乙醇提取 ,固定相氨基键合相色谱柱 ,流动相乙腈 水 (75∶25) ,检测波长 265nm。结果 :进样量 3.68～73.60μg·mL-1,r=0.9999,加样回收率 97.4% ,RSD 1.83% (n =6 )。结论 :可为胡芦巴的质量控制提供依据。 |
| 关 键 词： | 高效液相色谱法 胡芦巴 胡芦巴碱 |
| 文章编号： | 1001-5302(2002)03-0194-03 |
| 收稿时间： | 2001-07-08 |
| 修稿时间： | 2001-07-08 |
Determination of Trigonelline in Trigonella foenum-graecum by HPLC |
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| ZHAO Huai-qing ;QU Yan ;WANG Xue-ya ;ZHANG Hong-jian ;LI Fa-mei ;Hattori Masao. Determination of Trigonelline in Trigonella foenum-graecum by HPLC[J]. China Journal of Chinese Materia Medica, 2002, 27(3): 194-196 | |
| Authors: | ZHAO Huai-qing QU Yan WANG Xue-ya ZHANG Hong-jian LI Fa-mei Hattori Masao |
| Affiliation: | Shenyang Pharmaceutical University, Pharmaceutical College, Shenyang 110015, Liaoning, China. |
| Abstract: | OBJECTIVE: A HPLC method is established to determine the content of trigonelline in Trigonella foenum-graecum. METHOD: The medicinal material was extracted by petholeum ether-ethanol. Asahipak NH2P-50 column was used, mobilephase consisted of acetonitrile-water(75:25) and detection wavelength was set at UV 265 nm. RESULT: The standard curve was linear in the range of 3.68-73.60 micrograms.mL-1 with the correlation coefficient of 0.9999. The average recovery rate and RSD were 97.4% and 1.83% (n = 6) respectively. CONCLUSION: It provides scientific indexes for quality control of T. foenum-graecum. |
| Keywords: | HPLC Trigonella foenum-graecum trigonelline |
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