| 四神丸对溃疡性结肠炎小鼠外周血CD40,CD40L的调节作用 |
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| 引用本文： | 黄小英,赵海梅,管咏梅,万盼婷,童文婷,刘端勇. 四神丸对溃疡性结肠炎小鼠外周血CD40,CD40L的调节作用[J]. 时珍国医国药, 2011, 0(11): 2625-2627 |
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| 作者姓名： | 黄小英 赵海梅 管咏梅 万盼婷 童文婷 刘端勇 |
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| 作者单位： | 江西中医学院科研处;江西中医学院科技学院;江西中医学院现代中药制剂教育部重点实验室;江西中医学院; |
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| 基金项目： | 中药新型给药系统技术平台“十一五”重大新药创制项目(No.2008ZX09310-005); 江西省教育厅科学技术研究项目(No.GJJ09285); 江西省卫生厅中医科研计划(No.2007A0192009A0052009A101);江西省卫生厅科技计划(No.20092050) |
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| 摘 要： | 目的评价四神丸治疗小鼠结肠炎的疗效并探讨其对外周血CD40/CD40L的调节作用。方法昆明种小鼠60只,TNBS/乙醇法复制溃疡性结肠炎动物模型,随机分成正常组、四神丸高、中、低剂量组,柳氮磺吡啶(SASP)对照组,给药10d,观察小鼠结肠重量指数、病理变化及病理损伤评分以评价四神丸疗效,同时采用流式细胞术法检测结肠炎小鼠外周血CD40/CD40L的表达情况。结果模型组小鼠结肠重量指数明显高于正常组,并具有统计学意义(P<0.05),同时,各治疗小鼠结肠重量指数明显降低(P<0.05或P<0.01),而且模型组小鼠结肠病理损伤评分明显高于正常组(P<0.01),同时除高剂量组外,各治疗组小鼠结肠病理损伤评分明显低于模型组,且均有显著性统计学意义(P<0.05或P<0.01),治疗组镜下可见黏膜上皮完整或少量受损,炎细胞散在性分布,淋巴滤泡增生,基底层可见纤维结缔组织增生,溃疡修复。在CD40/CD40L表达方面,与正常组比较,模型组小鼠外周血细胞CD40分子表达明显偏高(P<0.05),而CD40L分子表达明显偏低(P<0.01),并具有统计学意义。各治疗组可见CD40分子表达较模型组明显降低(P<0.05或P<0.01),而CD40L分子表达则明显偏高并具有统计学意义(P<0.01)。结论四神丸可有效缓解结肠炎小鼠结肠损伤其作用途径可能与调节外周血CD40/CD40L信号有关。
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| 关 键 词： | 溃疡性结肠炎 四神丸 CD40/CD40L |
The Regulation Effect of Sishen Wan on the Expression of CD40,CD40l in Mice Peripheral Blood |
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| HUANG Xiao-ying,ZHAO Hai-mei,GUAN Yong-mei,WAN Pan-ting,TONG Wen-ting,LIU Duan-yong. The Regulation Effect of Sishen Wan on the Expression of CD40,CD40l in Mice Peripheral Blood[J]. Lishizhen Medicine and Materia Medica Research, 2011, 0(11): 2625-2627 |
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| Authors: | HUANG Xiao-ying ZHAO Hai-mei GUAN Yong-mei WAN Pan-ting TONG Wen-ting LIU Duan-yong |
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| Affiliation: | HUANG Xiao-ying1,ZHAO Hai-mei2,GUAN Yong-mei3,WAN Pan-ting4,TONG Wen-ting4,LIU Duan-yong 2* (1.Office of Scientific Research,Jiangxi College of TCM,Nangchang 330004,China,2.School of Science and Technology,Nanchang 330025,3.Key Lab of Modern TCM Preparation of State Educational Ministry,Nanchang 330004,4.Graduated Student of 2010,Nanchang,330004,China) |
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| Abstract: | Objective To evaluate the effect of SiShen Wan on expression of CD40,CD40L in peripheral blood from mice with experimental colitis. Methods Experimental colitis was induced by rectal administration of trinitrobenzene sulfonic acid(TNBS) dissolved in ethanol.The model animals were divided into four groups:the model control group,the high,middle,low dose of SiShen Wan treatment groups,and salicylazosulfapyridine treatment group.After 10-day treatment,the body weight,colonic wet weight,colonic weight index,col... |
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| Keywords: | Ulcerative colitis Sishen Wan CD40 CD40L |
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