青藏高原喜马拉雅旱獭鼠疫疫源地鼠疫耶尔森菌遗传特征分析

目的 研究青藏高原喜马拉雅鼠疫疫源地那曲和比如地区鼠疫耶尔森菌（鼠疫菌）菌株的分子分型特征，确定具有该分型特征的鼠疫菌在青藏高原鼠疫疫源地的分布情况。方法 应用板块重排、差异区段分析、间区规律短回文重复、多位点可变数目串联重复序列分析方法对分离自青藏高原地区、四川省、云南省的98株鼠疫菌进行分析。结果 那曲和比如地区的鼠疫菌菌株中第57~60板块的排列方式与测序菌株Z176003一致，在其他实验菌株中未发现该重排特征。结论 分离自那曲和比如的鼠疫菌菌株具有独特的分子分型特征，该特征在本地区的菌株中普遍存在，且菌株多态性持续分化。

Genetic analysis of Yersinia pestis strains isolated from Marmota himalayana plague focus of Qinghai-Tibet Plateau

Objective Identify the characteristics of genomovar of Yersinia pestis (Y. pestis)isolated from Naqu and Biru county in Tibet Autonomous Reagion and study the distribution of isolates with same genomovars characteristics in Qinghai-Tibet Plateau,including Tibet and Gansu,Sichuan,Yunnan provinces. Methods Analysis the molecular subtypes of 98 Y. pestis strains isolated in the plague focus of Qinghai-Tibet Plateau by 4 PCR-basic molecular subtyping methods,i.e.,Plate tectonics rearrangements,Different region (DFR),Clustered regularly interspaced short palindromic repeats (CRISPR),Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA). Results The rearrangement pattern of No.57-60 plate tectonics are the same in all Y. pestis strains isolated from Naqu and Biru county,just as the strains Z176003 (isolated in Naqu county and whole genomy was sequenced). No same genomy characterisctics were found in other strians from other regions in Qinghai-Tibet Plateau. Conclusion Strains isolated from Naqu and Biru county has its own characteristics of genome rearrangement and exclustive profiles of DRF and CRISPR. There were is a few different in VNTR loci of MLVA subtyping methods.