| 釉基质蛋白对牙周膜细胞增殖和碱性磷酸酶活性的影响 |
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| 引用本文： | 张丽青,万玲,吴织芬. 釉基质蛋白对牙周膜细胞增殖和碱性磷酸酶活性的影响[J]. 牙体牙髓牙周病学杂志, 2001, 11(4): 225-227 |
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| 作者姓名： | 张丽青 万玲 吴织芬 |
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| 作者单位： | 第四军医大学口腔医学院 |
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| 摘 要： | 目的：探讨釉基质蛋白对于牙周膜细胞增殖和碱性磷酸酶（ALP）活性的影响。方法：采用细胞培养技术，噻唑盐比色测定（MTT）法和酶动力学方法，观察釉基质蛋白对牙周膜细胞的作用。结果：釉基质蛋白组的牙周膜细胞，其增殖活性显著提高，以50mg/L浓度为最佳；其ALP活性也较对照组明显增加，以200mg/L浓度的效果最佳。具有一个合适的剂量范围。结论：釉基质蛋白可促进人牙周膜细胞的增殖和ALP活性。
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| 关 键 词： | 釉基质蛋白 牙周膜细胞 增殖 碱性磷酸酶 |
| 文章编号： | 1005-2593(2001)04-0225-03 |
| 修稿时间： | 2000-12-27 |
The effects of enamel matrix proteins on the proliferation and alkaline phosphotase activity of periodontal ligament cells |
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| ZHANG Li-qing,WAN Ling,WU ZHI-fen. The effects of enamel matrix proteins on the proliferation and alkaline phosphotase activity of periodontal ligament cells[J]. Chinese Journal of Conservative Dentistry, 2001, 11(4): 225-227 |
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| Authors: | ZHANG Li-qing WAN Ling WU ZHI-fen |
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| Abstract: | AIM: To observe the effects of enamel matrix proteins (EMPs) on the proliferation and ALP activity of periodontal ligament (PDL) cells. METHODS: Cell culture technique, MTT assay and enzyme kinetics methods were used. RESULTS: Both the proliferation and ALP activity of PDL cells in experimental groups were siganificantly increased and the best density of the former is 50mg/L and the latter is 200mg/L. CONCLUSIONS: EMPs can improve the proliferation and ALP activity of human PDL cells. [ |
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| Keywords: | periodontal ligament cells enamel matrix proteins proliferation alkaline phosphotase |
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