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探讨微阵列酶联免疫法在艾滋病哨点监测工作中的应用
引用本文:张建涛,徐志鑫,郝志波.探讨微阵列酶联免疫法在艾滋病哨点监测工作中的应用[J].中国卫生检验杂志,2011(12).
作者姓名:张建涛  徐志鑫  郝志波
作者单位:北京市昌平区疾病预防控制中心;北京华大吉比爱生物技术有限公司;
摘    要:目的:探讨微阵列酶联免疫法检测的临床价值。方法:用酶联免疫吸附试验(ELISA)和微阵列酶联免疫法(Ar-ray-ELISA)检测试验组样本的抗-HIV抗体、抗-TP抗体。分别统计两种方法检测阳性、阴性的样本数目,并进行统计学处理。结果:两种方法分别检测400份实验组样本,统计检测结果并经卡方检验证明无差别。检测结果的总符合率均为100%。结论:微阵列酶联免疫法与ELISA法无统计学差异,初步证明可以满足艾滋病哨点监测工作中检测工作的要求。

关 键 词:微阵列酶联免疫法  酶联免疫吸附试验  人类免疫缺陷病毒  梅毒螺旋体  

Study of microarray enzyme-linked immunosorbent assay in detection of pathogens in AIDS workstation
ZHANG Jian-tao,XU Zhi-xin,HAO Zhi-bo.Study of microarray enzyme-linked immunosorbent assay in detection of pathogens in AIDS workstation[J].Chinses Journal of Health Laboratory Technology,2011(12).
Authors:ZHANG Jian-tao  XU Zhi-xin  HAO Zhi-bo
Institution:ZHANG Jian-tao1,XU Zhi-xin1,HAO Zhi-bo2 (1.Changping District Center for Disease Control and Preveniton,Beijing 102200,China,2.Beijing BGI-GBI Biotech Co.,Ltd.,Beijing 101300,China)
Abstract:Objective:To investigate the clinical diagnostic value of the microarray enzyme-linked immunosorbent assay(Array-ELISA).Methods: Total samples were tested by enzyme-linked immunosorbent assay(ELISA) and Array-ELISA respectively for anti-HIV antibodies and anti-TP antibody.The amount of positive and negative samples obtained by the two methods were taken for statistical analysis.Results: 400 samples were tested by the two methods respectively,and there was no difference between two methods through the test o...
Keywords:Microarray-ELISA  ELISA  Human immunodeficiency virus  Treponema pallidum  
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