肺结核合并糖尿病患者外周血CD4~+CD25~+调节性T细胞的检测分析

目的:检测肺结核合并糖尿病患者外周血CD4+CD25+调节性T(Treg)细胞,探讨Treg细胞在疾病发生发展中的作用。方法:采用流式细胞技术多色荧光分析法,对81例肺结核合并糖尿病患者、63例肺结核患者及38例健康对照者外周血中Treg细胞测定,观察肺结核合并糖尿病患者与肺结核患者、健康对照者之间的差别。结果:肺结核合并糖尿病组与肺结核组外周血Treg细胞增多,两组间比较:t=2.24,P<0.05;肺结核合并糖尿病组与健康对照组外周血Treg细胞比较:t=3.79,P<0.01;肺结核组与健康对照组外周血Treg细胞比较:t=2.77,P<0.01。肺结核合并糖尿病初治组和复治组外周血Treg细胞比较:t=2.19,P<0.05;相关性分析显示肺结核合并糖尿病组中血糖浓度与Treg细胞数量之间呈正相关(r=0.269,P<0.01)。结论:肺结核合并糖尿病患者外周血Treg细胞数量增多,诱导免疫耐受,在疾病的发生发展中起到一定作用。

The investigation of the CD4~+CD25~+ regulatory T cells in peripheral blood from the patients with pulmonary tuberculosis related diabetes

Objective:To determine the percentages of CD4+ CD25+ regulatory T(Treg) cells in peripheral blood from the patients with pulmonary tuberculosis related diabetes,and to investigate the effect on disease development.Methods: A multi-color fluorescence flow cytometry analysis was employed to detect the percentages of Treg cells in peripheral blood from 81 patients with pulmonary tuberculosis related diabetes,63 patients with tuberculosis and 38 healthy donors;and the differences among the three were observed.R...