|
|||||
|
|
| 三氯乙烯变态反应病人外周血凋亡基因表达水平的研究 | |
| 引用本文： | 周丽,徐新云,刘月峰,黄新凤,杨细飞,毛吉炎,毛侃琅. 三氯乙烯变态反应病人外周血凋亡基因表达水平的研究[J]. 中国卫生检验杂志, 2011, 0(10): 2455-2457 |
| 作者姓名： | 周丽 徐新云 刘月峰 黄新凤 杨细飞 毛吉炎 毛侃琅 |
| 作者单位： | 广东省深圳市疾病预防控制中心; |
| 基金项目： | 深圳市科技计划项目资助课题(200802073) |
| 摘 要： | 目的:探讨三氯乙烯(TCE)变态反应病人外周血凋亡基因BAX、BAD、Bcl-2、Caspase-3、Caspase-8及Caspase-9的mRNA表达水平变化情况。方法:抽取健康人(对照组)和三氯乙烯变态反应病人(病例组)抗凝外周全血,采用实时荧光定量PCR(real-time fluorescent quantitative PCR)技术检测外周血中BAX、BAD、Bcl-2、Caspase-3、Caspase-8及Caspase-9的mRNA的表达水平。结果:TCE变态反应病人(病例组)BAX、BAD、Bcl-2基因mRNA表达水平与对照组相比较,分别升高85%1、39%、227%(P<0.01);病例组Caspase-3、Caspase-8、Caspase-9基因mRNA表达水平也比对照组分别升高130%、231%2、49%(P<0.01)。结论:三氯乙烯病人外周血凋亡基因表达水平明显高于对照组,提示这些凋亡基因可能与TCE引起的变态反应存在一定的关联。 |
| 关 键 词： | 三氯乙烯 变态反应 凋亡基因 实时荧光定量PCR |
Study on mRNA expression of apoptosis genes in patients with allergic disorder induced by trichloroethylene |
|
| ZHOU Li,XU Xin-yun,LIU Yue-feng,HUANG Xin-feng,YANG Xi-fei,MAO Ji-yan,MAO Kan-lang. Study on mRNA expression of apoptosis genes in patients with allergic disorder induced by trichloroethylene[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(10): 2455-2457 | |
| Authors: | ZHOU Li XU Xin-yun LIU Yue-feng HUANG Xin-feng YANG Xi-fei MAO Ji-yan MAO Kan-lang |
| Affiliation: | ZHOU Li,XU Xin-yun*,LIU Yue-feng,HUANG Xin-feng,YANG Xi-fei,MAO Ji-yan,MAO Kan-lang(Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,China) |
| Abstract: | Objective:To study mRNA expression of apoptosis genes BAX,BAD,Bcl-2,Caspase-3,Caspase-8,Caspase-9 in peripheral blood of patients with allergic disorder induced by trichloroethylene(TCE).Methods: Peripheral blood samples were collected from the healthy workers(control) and TCE patients.Real-time fluorescent quantitative PCR was used to detect mRNA expression of BAX,BAD,Bcl-2,Caspase-3,Caspase-8,Caspase-9.Results:The levels of BAX,BAD,Bcl-2 mRNA expression increased by 85%,139%,227%,respectively in TCE patie... |
| Keywords: | Trichloroethylene Allergy Apoptosis genes Real-time fluorescent quantitative PCR |
| 本文献已被 CNKI 等数据库收录！ | |