| 流式细胞术检测家具企业混苯接触工人DNA损伤 |
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| 引用本文： | 宣志强,黎君,赵尧贤,郦依华,吴寿明,俞顺飞.流式细胞术检测家具企业混苯接触工人DNA损伤[J].中国卫生检验杂志,2011(11):2662-2664. |
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| 作者姓名： | 宣志强 黎君 赵尧贤 郦依华 吴寿明 俞顺飞 |
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| 作者单位： | 浙江省疾病预防控制中心;浙江大学医学院环境医学研究所; |
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| 基金项目： | 浙江省医药卫生科学研究基金项目(2009A038) |
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| 摘 要： | 目的:研究家具企业混苯作业工人外周血DNA损伤。方法:应用流式细胞术对32名混苯接触组和30名餐饮业对照组人员外周血淋巴细胞DNA损伤进行检测。结果:混苯接触组工人外周血淋巴细胞DNA损伤率和几何平均荧光强度均高于对照组(P<0.01),而不同工龄组工人DNA损伤率和几何平均荧光强度差别无统计学意义(P>0.05)。结论:职业性混苯接触可引起人外周血淋巴细胞DNA损伤,流式细胞术γH2AX分析法是一种有应用前景的检测外周血淋巴细胞DNA损伤的方法。
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| 关 键 词： | 流式细胞术 混苯 淋巴细胞 DNA损伤 |
Detection of DNA damage in workers occupationally exposed to mixed benzene with flow cytometry |
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| XUAN Zhi-qiang,LI Jun,ZHAO Yao-xian,LI Yi-hua,WU Shou-ming,YU Shun-fei.Detection of DNA damage in workers occupationally exposed to mixed benzene with flow cytometry[J].Chinses Journal of Health Laboratory Technology,2011(11):2662-2664. |
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| Authors: | XUAN Zhi-qiang LI Jun ZHAO Yao-xian LI Yi-hua WU Shou-ming YU Shun-fei |
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| Institution: | XUAN Zhi-qiang1,LI Jun2,ZHAO Yao-xian1,LI Yi-hua1,WU Shou-ming1,YU Shun-fei1(1.Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 310051,China,2.Environmental Medicine Institute of Zhejiang University School of Medicine,Hangzhou 310058,China) |
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| Abstract: | Objective:To study DNA damage of peripheral blood lymphocytes in workers from furniture industry occupationally exposed to mixed benzene with flow cytometry(FCM) assay.Methods: FCM was used to detect DNA damage of peripheral blood lymphocytes obtained from 32 workers exposed to mixed benzene(exposure group) and 30 food processing workers(control group).Results: DNA damage rate and geometric mean fluorescence intensity in peripheral blood lymphocytes of exposure group were significantly higher than that of c... |
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| Keywords: | Flow cytometry Mixed benzene Lymphocyte DNA damage |
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