喘可治注射液引起的过敏反应研究

目的建立喘可治注射液引起过敏反应的快速检测方法。方法采用1次.d 1、连续3 d静脉注射药物致敏豚鼠,第8天以ELISA法测定血清IL-4和总IgE;并与传统的腹腔注射致敏检查方法相比较。结果 3批喘可治注射液本身不引起豚鼠过敏反应,但添加1.5 g.L 1绿原酸后进行致敏第8天后豚鼠血清IL-4和总IgE均明显升高,豚鼠出现了过敏反应,时间比传统检查方法提早1~2周,且二者结果一致。结论所建立的新方法可用于快速检测喘可治注射液引起的过敏反应。

Study on Allergic Reaction Induced by Chuankezhi Injection

OBJECTIVE To establish a method of rapid test for allergic reaction induced by Chuankezhi injection（CI）. METHODS The guinea pigs were sensitized continuously by intravenous injection of drugs once a day for three days, IL-4 and total IgE of serum were detected with ELISA method on the 8th day. The results were compared with traditional method of sensitization by intraperitoneal injection. RESULTS The allergic reaction didn＇t occur on the 8th day after guinea pigs were sensitized by intravenous injection of three batches of CI, but IL-4 and total IgE of serum increased obviously and allergic reaction occurred from the 8th day after sensitized by CI added with 1.5 g.L-1 chlorogenic acid, the onset time of allergy was reduced by 1-2 weeks as compared with traditional method, and the results were in accordance with each other. CONCLUSION It provides a new way of rapid test for CI induced allergic reaction.