| 检测Epstein—Barr病毒特异性细胞毒性T淋巴细胞方？… |
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| 引用本文： | 刘海鹰,周玲.检测Epstein—Barr病毒特异性细胞毒性T淋巴细胞方？…[J].中华实验和临床病毒学杂志,1998,12(4):357-360. |
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| 作者姓名： | 刘海鹰 周玲 |
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| 摘 要： | 目的 建立一种非放射性、简便易行的可检测特异性细胞毒性Ｔ淋巴细胞的方法，并且初步应用于Ｅｐｓｔｅｉｎ－Ｂａｒｒ病毒的细胞免疫应答。方法 用重组的ＥＢＶ－ＬＭＰ１痘苗病毒、ＴＫ＾＋痘苗病毒和杆状病毒系统表达的ＥＢＶ－ＬＭＰ！蛋白分别免疫Ｂａｌｂ／Ｃ小鼠，用Ｐ８１５细胞和乳酸脱氢酶法检测ＥＢ病毒特异性细胞毒性Ｔ细胞的杀伤效应。结果 重组ＥＢＶ－ＬＭＰ１痘苗病毒免疫组原发ＣＴＬ水平和体外诱生的二次ＣＴＬ
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| 关 键 词： | 特异性细胞毒性 T淋巴细胞 EB病毒 乳酸脱氢酶 |
Establishment and primary study of an assay for the detection of EB virus specific cytotoxic T lymphocytes] |
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| H Liu,L Zhou,Y Zeng.Establishment and primary study of an assay for the detection of EB virus specific cytotoxic T lymphocytes][J].Chinese Journal of Experimental and Clinical Virology,1998,12(4):357-360. |
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| Authors: | H Liu L Zhou Y Zeng |
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| Institution: | Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052. |
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| Abstract: | In our study, a non-radioactive cytotoxicity assay by using a Balb/C (H-2d) mice, P815(H-2d) cells and lactate dehydrogenase (LDH) system was established, in order to detect EB virus specific cytotoxic T lymphocytes(CTLs). In this study, we detected both the changes of primary CTLs and secondary stimulated CTLs. Spleen CTL activity assay showed that the levels of primary and secondary CTLs in recombinant EBV-LMP1 vaccinia virus group were more higher than that in control group, at the same time, obvious enhancement of EBV-LMP1 specific CTL activity was observed in experimental group immunized by Baculovirus system expressing EBV-LMP1 protein when compared with the normal mice group. This work proved that the LDH assay can be used to detect cytotoxic T lymphocytes. |
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