| HPLC法测定三七中人参皂苷Rg1和三七皂苷R1的含量 |
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| 引用本文： | 张雯洁,李忠琼,张赟华,胡旭佳.HPLC法测定三七中人参皂苷Rg1和三七皂苷R1的含量[J].中国药品标准,2003,4(4):35-37. |
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| 作者姓名： | 张雯洁 李忠琼 张赟华 胡旭佳 |
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| 作者单位： | 云南省药品检验所,昆明,650011 |
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| 摘 要： | 目的：建立HPLC测定三七中人参皂苷Rg1和三七皂苷R1含量的方法。方法：色谱条件为：C18柱，流动相为乙腈-0．05％磷酸溶液(21．5：78．5)，UV检测波长为203nm。结果：此方法线性关系良好，平均加样回收率分别为人爹皂苷Rg199．54％和三七皂苷R1101．40％(n=6)。结论：本方法分离良好，结果准确可靠。
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| 关 键 词： | HPLC法 含量测定 三七 人参皂苷Rg1 三七皂苷R1 |
Determination of Ginsenoside Rg1 and Notoginsenoside R1 in Notoginseng by HPLC |
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| Authors: | Zhang Wenjie Li Zhongqiong Zhang Yunhua and Hu Xujia |
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| Institution: | Yunnan institute for Drug Control, Kunming 650011;Yunnan institute for Drug Control, Kunming 650011;Yunnan institute for Drug Control, Kunming 650011;Yunnan institute for Drug Control, Kunming 650011 |
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| Abstract: | Objective: To establish the determination method of Ginsenoside Kg1 and Notoginsenoside R1 in Notoginseng.Method: The HPLC method for determination of Notoginseng was.C18 column(250mm×4.6mm,5um),a mixture acetonitrile-0.05%phosphoric acid(21.5:78.5)as a mobile phase,detection wavelength 203nm.Results: This method showed a good linear relationship.The mean recovery was Ginsenoside Rg1 99.54% and Notoginsenoside R1 101.40%(n=6).Conclusion: This method showed a good separating degree and reliable. |
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| Keywords: | HPLC Ginsenoside Rg1 Notoginsenoside R1 Notoginseng |
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