鳖甲药材指纹图谱与其抗肝纤维化作用的谱效关系研究

目的研究不同产地鳖甲药材的HPLC指纹图谱与其抗纤维化作用的谱效关系,揭示鳖甲药材抗肝纤维化作用的"药效成分组"。方法 HPLC-DAD法测定了12个产地鳖甲药材样品,采用中药色谱指纹图谱相似度评价系统2004 A进行评价,建立了共有模式,并进行主成分分析(PCA)。以LX-2肝星状细胞为模型研究其对肝细胞的抑制效果,采用SPSS软件对数据进行相关分析,研究谱效相关性。结果 12个产地鳖甲药材HPLC指纹图谱中共有7个特征共有峰,其中峰2、5与LX-2肝星状细胞抑制效果(A值)的相关性呈现中等程度相关,峰4的相关性最强。由PCA可知,峰4的量对鳖甲质量有着重要的影响。结论 12个产地鳖甲药材对LX-2肝星状细胞均有抑制作用,HPLC指纹图谱与其抗纤维化作用之间有一定的相关性,为进一步控制鳖甲药材内在质量提供参考。

Spectrum-activity relationship on anti-hepatic fibrosis efficacy of Trionycis Carapax

Objective To investigate the spectrum-activity relationship between HPLC of Trionycis Carapax from different regions and its anti-hepatic fibrosis efficacy, in order to reveal the "active component group" for anti-hepatic fibrosis efficacy of Trionycis Carapax. Methods The samples from 12 regions were determined with the HPLC-DAD method. The representative standard fingerprint was calculated using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A). The common patten and the principal component analysis (PCA) were established. Using the LX-2 hepatic stellate cell as a hepatic fibrosis model of Trionycis Carapax to study its inhibition on the hepatic fibrosis cells. The spectrum-effect relationship was studied by SPSS 19.0 software. Results Seven common peaks in the HPLC-fingerprint of Trionycis Carapax were obtained. It was tentatively concluded that peaks 2 and 5 related better to the inhibitory effect of LX-2 cells (OD value) in the seven characteristic peaks. Peak 4 had the strongest correlation. And the quality of Trionycis Carapax was influenced by the concentration of peak 4 obtained by PCA. Conclusion All the samples could inhibit the proliferation of LX-2 hepatic stellate cell in some extent. There may be a certain relationship between HPLC fingerprint and anti-hepatic fibrosis efficacy. In addition, the research could be used as the quality control method of Trionycis Carapax.