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| 黑老虎果皮体外抑菌活性的初步研究 | |
| 引用本文： | 封毅,李志春,孙健,卓少元,范蓉,乐宁,尧金燕,龙兴.黑老虎果皮体外抑菌活性的初步研究[J].时珍国医国药,2011,22(4):822-824. |
| 作者姓名： | 封毅 李志春 孙健 卓少元 范蓉 乐宁 尧金燕 龙兴 |
| 作者单位： | 1. 广西中医学院基础医学院,广西,南宁,530001 2. 广西农业科学院农产品加工研究所,广西,南宁,530007 3. 广西农业科学院园艺研究所,广西,南宁,530007 |
| 基金项目： | 广西壮族自治区自然科学基金，广西壮族自治区农业科学院博士启动基金 |
| 摘 要： | 目的研究黑老虎果皮及其提取物的体外抑菌效果,确定黑老虎果皮对伤寒沙门氏菌的最小抑菌浓度、最小杀菌浓度和有效部位。方法采用滤纸扩散法观察黑老虎果皮及其乙醇提取液和各相萃取液的抑菌作用;用二倍稀释法测定最小抑菌浓度和最小杀菌浓度。结果黑老虎果皮及其乙醇提取物对伤寒沙门氏菌有抑制作用,最小抑菌浓度为31.25mg/ml,最小杀菌浓度为125 mg/ml,抑菌物质主要存在于乙醚萃取相中。结论黑老虎果皮对伤寒沙门氏菌有显著地抑制作用。 |
| 关 键 词： | 黑老虎 果皮 抑菌作用 伤寒沙门氏菌 |
Antibacterial Activities of Peels of Kadsura coccinea(Lem.)A.C.Smith in vitro |
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| FENG Yi,LI Zhi-chun,SUN Jian,ZHUO Shao-yuan,FAN Rong,LE Ning,YAO Jin-yan,LONG Xing.Antibacterial Activities of Peels of Kadsura coccinea(Lem.)A.C.Smith in vitro[J].Lishizhen Medicine and Materia Medica Research,2011,22(4):822-824. | |
| Authors: | FENG Yi LI Zhi-chun SUN Jian ZHUO Shao-yuan FAN Rong LE Ning YAO Jin-yan LONG Xing |
| Institution: | 1.Faculty of Preclinical Medicine,Guangxi Traditional Chinese Medical University,Nanning 530001 China;2.Institute of Agri-food Science & Technology,Guangxi Academy of Agricultural Sciences,Nanning 530007 China;3.Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China;Corresponding author) |
| Abstract: | Objective To determine the antimicrobial activities of Kadsura coccinea peels against several human pathogenic bacteria and fungus in vitro.Methods Paper disc method was conducted to investigate the antibacterial activities of Kadsura coccinea peels.Broth dilution assay was employed for the measurements of minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of the ethanol extract of the peels.Results Kadsura coccinea peels showed stable antibacterial activity on Salmonella typhi at temperatures no more than 100 ℃.MIC and MBC of the ethanol extract of the peels toward Salmonella typhi were 31.25 mg/ml and 125 mg/ml,respectively.The antibacterial substance mainly existed in the ethyl ether fraction from the ethanol extract of the peels.Conclusion Kadsura coccinea peels possess significant antibacterial activity against Salmonella typhi. |
| Keywords: | Kadsura coccinea(Lem ) A C Smith peels Antibacterial activity Salmonella typhi |
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