细胞凋亡相关斑点样蛋白与半胱氨酸蛋白酶-1在肝癌中的表达及临床意义

目的分析细胞凋亡相关的斑点样蛋白（ASC）与半胱氨酸蛋白酶-1（Caspase-1）在肝癌与癌旁组织中的表达及临床意义。方法对30例术前未使用放疗与化疗的肝癌与癌旁组织标本采用免疫组化LSAB法进行ASC、Caspase-1测定,并对各指标进行分析。结果ASC阳性率在肝癌与癌旁组织中分别为10．00％（3／30）和73．33％（22／30）,组间阳性表达率差异有统计学意义（P〈0．01）;Caspase．1阳性率分别为23．33％（7／30）和66．67％（20／30）,组间阳性表达率差异有统计学意义（P〈0．01）。ASC与Caspase-1两指标在癌组织中的表达均降低,两者不相关（P〉0．05）,在癌旁组织中表达均显著增高,两者呈正相关（r=0．722,P〈0．01）。结论ASC、Caspase-1在肝癌中表达低,且显著低于癌旁组织,提示ASC、Caspase-1与肝癌的凋亡、发生及发展过程密切相关,可作为肝癌的诊断及治疗有希望的靶点。

The expression of ASC and Caspase-1 in liver cancer and its clinic significance

Objective To analyze the apoptosis-related spots like protein (ASC) and caspase -1 in HCC and adjacent noncancerous tissues and its clinical significance. Methods ASC and Caspase 1 were determined with immunohistochemistry LSAB method and the various indexes were analyzed in 30 cases of liver cancer and adjacent tissues which did not receive preoperative radiotherapy and chemotherapy. Results The positive rate of ASC in HCC and adjacent noncancerous tissues was 10.00% (3/30) and 73.33% (22/30). Positive rate between the groups had significant difference (P＜0.01). Caspase-1 positive rate had significantly difference between the groups (23.33% (7/30) vs. 66.67% (20/30), P＜0.01). ASC and the two indicators of Caspase-1 expression in cancer tissues were low, but no significant correlations between the two groups (P＞0.05). ASC and the two indicators of Caspase-1 expression in adjacent tissue were significantly increased, and it showed a very significant correlation (r＝0.722,P＜0.01). Conclusions ASC, Caspase-1 expression in liver cancer were low expressed, and significantly lower than the adjacent tissues, which suggested that ASC and Caspase-1 were closely related to the occurrence and development of the apoptosis of liver cancer, and it might be used as diagnosis and treatment of targets in liver cancer.