益气回阳注射液对心肺复苏后大鼠血及心肌ICAM-1表达的影响

目的：研究益气回阳注射液对大鼠缺氧型心脏骤停模型（CA）心肺复苏（CPR）后血及心肌细胞黏附分子-1（ICAM-1）表达的影响。方法：将50只清洁级雄性Wistar大鼠随机分为空白组、模型组、肾上腺素组、益气回阳组和肾上腺素＋益气回阳组，每组10只；采用夹闭气管窒息法制作大鼠心脏骤停动物模型，造模成功后根据不同分组给予不同药物，从股静脉内注射，每5min一次，直至复苏成功，或复苏时间达到30min为止，流式细胞仪（FCM）检测血白细胞及心肌细胞ICAM-1表达量。结果：模型组ICAM-1的表达与空白组比较有显著性差异（P〈0．05）；肾上腺素组、益气回阳组以及肾上腺素＋益气回阳组血、心肌组织ICAM-1的表达受抑制，与模型组比较均有显著性差异（P〈0．05），组间比较无差别（P〉0．05）。结论：益气回阳注射液可能通过抑制血白细胞及心肌ICAM-1表达，在一定程度上减轻因复苏引起的心肌细胞的损伤。

Study on Yiqi Huiyang Injection(益气回阳注射液) on Express of ICAM-1 of Mouse Blood Cell and Cadiocyte after Resuscitation

Objective:To study the effect of Yiqi Huiyang Injection (YHI) on express of ICAM-1 of blood cell and cadiocyte of model mouse after resuscitation from cardiac arrest (CA).Methods:Fifty Wistar rats were divided randomly into five groups,the control group,the model group,the adrenalin group,the YHI group,the combined adrenalin and YHI group.The express of ICAM-1 were determined with flow cytometor.Result:The difference of express of ICAM-1 between the model group and the control group was significant (P<0.05). The difference of express of ICAM-1 between the control group and the adrenalin group,the YHI group,combined adrenalin and YHI group was significant (P<0.05). Conclusion:YHI had the effect of reducing the myocardial injury by blocking the express of ICAM-1.