日本血吸虫感染者尿液中特异性IgG及亚类的检测

目的 研究日本血吸虫感染者尿液特异性循环抗体在日本血吸虫诊断上的应用。方法 采集类检确诊的日本血吸虫患者尿液，ELISA法测定尿液中抗日本血吸虫特异性IgG及亚类（IgG1，IgG2，IgG3）抗体；同时肝吸虫阳性患者尿液作为考核交叉反应样本，无寄生虫感染液作为阴性对照。结果 粪检阳性日本血吸虫患者尿液中抗日本血吸虫IgG抗体的检出率为81.0%（17/21），其中IgG1为81.0%（17/21），IgG2为19.0%（4/21），IgG3为23.8%（5/21），肝吸虫患者尿液中IgG1抗体与日本血吸虫抗原有25.0%（2/8）的交叉反应，IgG2和IgG3均未发生交叉反应。结论 日本血吸虫患者尿液中存在抗日本血吸虫的特异性循环抗体，肝吸虫患者尿液中的IgG1抗体与日本血吸虫抗原有一定的交叉反应；但IgG2和IgG3无交叉反应现象，提示尿液中的IgG抗体可以作为血吸虫感染的诊断，尿液中的IgG2和IgG3抗体可以作为日本血吸虫病的鉴别诊断。

Detection of IgG antibody and subclass from urine of patients infected with Schistosoma japonicum

Objective To detect specific circular antibody from the urine of patients infected with Schistosoma japonicum (S japonicum) and study its application in diagnosis of schistosomiasis Methods The urine of patients with S japonicum was collected, IgG antibody and its subclass (IgG 1,IgG 2,IgG 3) were detected by ELISA, the urine of patients with Clonorchis sinensis was used as cross reaction control and normal urine as negative control Results The detection rate of IgG specific antibody in urine of patients with S japonicum was 81 0% (17/21),and IgG 1,IgG 2, IgG 3 were 81 0% (17/21), 19 0% (4/21) and 23 8% (5/21), respectively; a 25 0% (2/8) cross reaction of the IgG 1 antibody in urine of patients with Clonorchis sinensis with S japonicum antigen was observed But no reaction was observed with IgG 2,and IgG 3 antibody Conclusion There were anti- S japonicum antibodies in the urine of patients with S japonicum The IgG 1 antibody in urine of patients with Clonorchis sinensis was reacted with S japonicum antigen,suggesting that IgG antibody can be detected from the urine of schistosomiasis patients and IgG 2, and IgG 3 in the urine of shcistosomiasis patients can be used for differential diagnosis of shcistosomiasis