Bcl-2、 cyclin A2和P13K在激素受体阴性乳腺癌中的表达及意义 |
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引用本文: | 王敏,李惠平,赵红梅,雷玉涛,王丽娜,王晶,刘雅昕.Bcl-2、 cyclin A2和P13K在激素受体阴性乳腺癌中的表达及意义[J].中国微创外科杂志,2013(8):713-716,723. |
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作者姓名: | 王敏 李惠平 赵红梅 雷玉涛 王丽娜 王晶 刘雅昕 |
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作者单位: | [1]北京大学肿瘤医院暨北京市肿瘤防治研究所乳腺内科恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142 [2]北京大学第三医院普外科,北京100191 |
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摘 要: | 目的探讨激素受体阴性乳腺癌中B淋巴细胞瘤/白血病2基因( B-cell lymphoma/leukemia-2, Bcl-2 )、细胞周期素A2(cyclinA2)和磷酸肌醇3激酶( phosphoinositide 3 -kinase, PI3K )的表达及意义。方法采用实时聚合酶链反应(real time polymerase chain reaction,RT-PCR)方法检测Bcl-2、cyclinA2和P13K在激素受体阴性乳腺癌组织(58例)、乳腺正常组织(14例)中的表达水平,并采用Mann-WhitneyU检验分析其与临床病理特征的关系。结果与乳腺正常组织相比,激素受体阴性乳腺癌中Bel-2表达降低,而cyclinA2和P13K表达增高(P〈0.05)。在乳腺癌组织中,Bcl-2在TNM分期Ⅲ期、组织学分级Ⅲ级和出现转移复发的患者中表达水平降低(P〈0.05),但其表达在不同年龄、肿瘤大小和淋巴结转移的组间差异无显著性(P〉0.05);cyclinA2在转移复发的患者中显著高表达(P〈0.05),但在不同的年龄、肿瘤大小、组织学分级、TNM分期和淋巴结状态的组间差异均无显著性(P〉0.05);P13K在有淋巴结转移和TNM分期Ⅲ期的患者中高表达(P〈0.05),但其表达在不同年龄、肿瘤大小、组织学分级以及是否转移复发的组间差异均无显著性(P〉0.05)。结论Bcl-2低表达、eyclinA2和P13K高表达可能与激素受体阴性乳腺癌的发生发展有关。
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关 键 词: | 激素受体阴性乳腺癌 B淋巴细胞瘤 白血病2基因 细胞周期素A2 磷酸肌醇3激酶 实时聚合酶 链反应 |
The Expression of Bci-2, Cyclin A2 and PI3K in Hormone Receptor Negative Breast Cancer and Their Significance |
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Institution: | Wang Min , Li Huiping, Zhao Hongmei, et al.( Department of Breast Oncology, Peking University Caner Hospital & Institute, Key Laboratory of Carcinogenesis and Translational Research ( Ministry of Education), Beijing 100142, China) |
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Abstract: | Objective To study the expression of Bcl-2, cyclin A2 and PI3K in hormone receptor negative breast cancer and their clinical significance. Methods Bcl-2, cyclin A2 and PI3K amplifications were analyzed using real time PCR in hormone receptor negative breast cancer (58 cases ) and normal breast tissues (14 cases ). Their relationship with clinicopathological characteristics were analyzed using Mann-Whitney U test. Results Compared with normal breast tissue, the expression of Bcl-2 decreased in hormone receptor negative breast cancer, while the expression of cyclin A2 and PI3K increased (P 〈 0.05 ). Bcl-2 expression in patients with stage Ⅲ, grade Ⅲ or tumor metastasis group were significantly lower ( P 〈 0.05 ). But there was no association with age, tumor size and lymph node status ( P 〉 0. 05). The high expression of cyclin A2 was observed in patients with metastasis or recurrence( P 〈 0. 05 ). The expression of cyclin A2 was not significantly different in different groups in terms of age, tumor size, grade, lymph node status and stage(P 〉 0.05). PI3K expression was significantly higher in the patients with lymph node metastasis or stage Ⅲ ( P 〈 0. 05). But there was no association with age, tumor size, grade or metastasis ( P 〉 0. 05 ). Conclusion Bcl-2 low expression, cyclin A2 and PI3K high expression might be closely correlated with the occurrence and development of hormone receptor negative breast cancer. |
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Keywords: | Hormone receptor negative breast cancer Bcl-2 Cyclin A2 PI3K Real time PCR |
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