不同入肝血流阻断方式对小鼠肝癌生长和转移的影响

目的探讨不同血流阻断方式对肝癌生长和转移的影响。方法选择昆明小鼠24只,随机分为正常对照组(suspended operation,SO)、肝门阻断组(occlusion of the portal triad,OPT)、保留肝动脉持续阻断门静脉组(occlusion of portal vein,OPV)各8只。采用门静脉注射肿瘤的方法建立肝癌模型,建模后3d采用阻断范围为左外叶和中叶、阻断时间为60min的入肝血流阻断方式,复流后5d分别对三组动物测定肝脏置换区域(HRA)、增殖性细胞核抗原(PCNA)在肝癌组织中的阳性表达率。结果门静脉注射小鼠肝癌细胞8d后,对照组阻断叶HRA(%)值为7.658±2.552,OPT组升高到35.612±4.234,OPV组升高到9.02±3.006(P<0.01);对照组非阻断叶HRA(%)值为8.107±2.003,OPT组升高到8.698±3.021,OPV组升高到8.607±2.304(P<0.01)。对照组中阻断叶与非阻断叶肿瘤生长比率为1,OPT组值为2.82,OPV组值为1.1。对照组中肿瘤细胞PCNA阳性表达率为30%,OPT组为78%,OPV组为45%。结论保留肝动脉持续阻断门静脉可减缓肝癌的生长和转移。

Effect of different hepatic blood flow occlusion procedures on growth and metastasis of liver cancer in mice

Objective To study the effect of different hepatic vascular occlusion procedures on growth and metastasis of liver cancer.Methods Twenty-four Kunming mice were randomly divided into suspended operation(SO) group,sham operation group,occlusion of portal triad(OPT) group,and occlusion of portal vein(OPV) group(8 in each group).Three days after a hepatocellular carcinoma(HCC) model was established via portal vein injection of HCC cells,both portal vein and hepatic artery were clamped or portal vein to the median and left liver lobes was selectively clamped for 60 minutes.Five days after reperfusion,expression rates of proliferative cell nuclear antigen(PCNA) in hepatic replacement area(HRA) and in HCC tissues were measured.Results Eight days after injection of HCC cells from mice via the portal vein,the value of HRA with hepatic lobe occlusion was 7.658±2.552,35.612±4.234 and 9.02±3.006 in control group,OPT group and OPV group,respectively(P<0.01).The value of HRA without hepatic lobe occlusion was 8.107±2.003,8.698±3.021 and 8.607±2.304 in control group,OPT group and OPV group,respectively(P<0.01).The tumor growth ratio between hepatic lobe occlusion and non hepatic lobe occlusion in HRA was 1,2.82 and 1.1 in control group,OPT group and OPV group,respectively.The positive expression rate of PCNA in HCC tissues was 30%,78% and 45% in control group,OPT group and OPV group,respectively.Conclusion Preservation of hepatic artery flow during portal triad blood inflow occlusion can slow down the growth and metastasis of liver cancer in mice.