山东地区5 844例脐血HLA I类等位基因多态性分析

目的:研究山东地区脐带血HLA I类(HLA-A,-B)等位基因的分布特征,探讨中国其他地区及世界不同种族利用山东脐血供者进行造血干细胞移植的可能性。方法:应用PCR-SSO方法对山东地区5 844例无血缘关系的汉族健康新生儿脐带血进行了HLA-A,-B等位基因的分布调查。结果:HLA-A等位基因,检出20种;HLA-B等位基因,检出46种。通过与不同地域汉族人群的比较显示:在不同种族的人群中有其各自独特的人群分布特征,在不同地域的同一民族人群中亦有其独特的地理分布特征。结论:山东脐血HLA I类基因具有多态性,能够代表山东汉族人群HLA的分布特征,北方人在山东脐血库中最容易寻找到HLA I类等位基因相合的异基因脐血供者,而南方人找到的机率较北方人稍低。

The allele polymorphisms of HLA class I (HLA-A and -B )of the umbilical cord blood of 5 844 Shandong people

Objective: To investigate the HLA-A and -B allele polymorphisms in the umbilical cord blood of Shandong people.Methods: 5 844 umbilical cord blood samples were drawn from the Shandong umbilical cord bank and determined by the polymerase chain reaction-sequence specific oligo-nucleotide(PCR-SSO) method. Results: Twenty alleles at HLA-A locus and forty-six alleles at HLA-B locus were detected.Of the twenty HLA-A alleles,the most prevalent five alleles were A*02(0.3041),A*11(0.144 3),A*24(0.143 4),A*30(0.097 5) and A*33(0.085 9), and the least prevalent alleles were A*34(0.000 6),A*25(0.000 5,A*66(0.000 5),A*74(0.000 4) and A*36(0.000 1).Of the forty-six HLA-B alleles,the most prevalent five alleles were B*13(0.134 8),B*51(0.071 3),B*62(0.071 2),B*61(0.067 6)and B*60(0.064 2),and the least prevalent alleles were HLA-B*77(0.000 1),B*76(0.000 2),B*47(0.000 3),B*42(0.000 3)and B*72(0.000 4).Comparing the HLA-A and-B gene frequencies of Shandong umbilical cord blood samples with those of other Han Chinese,there were unique distribution features of the HLA-A and-B alleles among the populations studied from various regions with the same racial origin.The differences from various regions of the same race were fewer than those from different races. Conclusion: The HLA-A and-B alleles in Shandong umbilical cord blood have a rich polymorphism,which represents the distribution characters of the HLA-A and-B alleles in Shandong Hans.For a patient of northern Han Chinese it is easy to search for a HLA-A or-B matched cord blood donor from the Shandong umbilical cord blood bank.For a patient of southern Chinese it is possibleto receive a transplant of cord blood stem cells matched with HLA-A or B from the Shandong umbilical cord blood bank.