| 粉防己碱对大鼠肺动脉平滑肌细胞小电导钙激活钾通道的作用 |
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| 引用本文： | 王中峰,开丽,肖欣荣. 粉防己碱对大鼠肺动脉平滑肌细胞小电导钙激活钾通道的作用[J]. 中国药理学通报, 2000, 16(2): 199-201 |
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| 作者姓名： | 王中峰 开丽 肖欣荣 |
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| 作者单位： | 第三军医大学生理学教研室!重庆400038中国科学院上海生理研究所，上海200031，第三军医大学大坪医院野战外科研究所!重庆400042，华西医科大学附一院呼吸内科!成都610041 |
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| 摘 要： | 目的 研究粉防己碱 (Tet)对大鼠肺动脉平滑肌细胞小电导钙激活钾通道 (SKCa)的作用。方法 内面朝外膜片箝单通道记录法。结果 Tet 7 5 μmol·L-1对电导值为 10pS的SKCa无明显影响 ,15 μmol·L-1可增加SKCa开放的概率 ,改变通道的开放和关闭模式 ,30 μmol·L-1降低通道的开放概率 ,通道开放以短暂簇状为主。结论 Tet对肺动脉平滑肌SKCa的作用与Tet的浓度有关 ,合适浓度下可增加通道的开放 ,K+ 外流增多 ,与Tet降低肺动脉张力有关。
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| 关 键 词： | 粉防己碱 钾通道 钙 膜片箝技术 平滑肌 肺动脉 大鼠 |
Effects of tetrandrine on small-conductance calcium activated potassium channels in rat pulmonary artery smooth muscle cells |
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| WANG Zhong Feng ,KAI Li ,XIAO Xin Rong. Effects of tetrandrine on small-conductance calcium activated potassium channels in rat pulmonary artery smooth muscle cells[J]. Chinese Pharmacological Bulletin, 2000, 16(2): 199-201 |
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| Authors: | WANG Zhong Feng KAI Li XIAO Xin Rong |
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| Affiliation: | WANG Zhong Feng 1,KAI Li 2,XIAO Xin Rong 3 |
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| Abstract: | AIM To study the effects of tetrandrine (Tet) on small conductance calcium activated potassium channels (SK Ca ) of rat pulmonary artery smooth muscle cells. METHODS Inside out configuration of patch clamp technique. RESULTS The conductance of SK Ca in our experiment was 10 pS. Tet 7 5 μmol·L -1 has not significant influence on the SK Ca , but 15 μmol·L -1 increased the open probability, changed the open and close model. When concentration of Tet rasied to 30 μmol·L -1 , it decreased the open probability and open time constants. CONCLUSION In appropriate concentration, Tet activated the SK Ca in pulmonary artery smooth muscle cells, which resulted in K + outflow increasing and the pulmonary vasoconstriction reduction. |
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| Keywords: | tetrandrine potassium channel calcium patch clamp technique smooth muscle artery rat |
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