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固相反向间接血凝试验检测抗-HBS的抗独特型抗体
引用本文:何东风,朱燕,朱沛轩,刘武. 固相反向间接血凝试验检测抗-HBS的抗独特型抗体[J]. 川北医学院学报, 1991, 0(2)
作者姓名:何东风  朱燕  朱沛轩  刘武
作者单位:四川省南充地区妇幼保健院,四川省南充地区妇幼保健院,四川省卫生管理干部学院,四川省卫生管理干部学院
摘    要:
本文建立了固相反向间接血凝检测抗-HBS 的抗独特型抗体的方法。该方法是用羊抗人 IgG 包被聚苯乙稀微孔板(V型),捕捉血清中的抗-HBS 的抗独特型抗体(IgG),再加抗-HBS 致敏的血球,观察其凝集结果。用本法对正常人血清际本116例以及 HBsAg 阳性血清标本102例进行了抗-HBS 的抗独特型抗体检测。在 HBsAg 阳性血清标本中共检出抗-HBS 的抗独特型抗体阳型标本24例,阳性率为23.7%。实验表明,该方法具有简便、准确、快速等优点,又不需要特殊设备,适合于各级医疗单位。

关 键 词:固相反  间接血凝  抗独特型抗体  抗-HBs

A SOLID PHASE LEVERSE PASSIVE HEMAGGLUTINATI-ON FOR THE DETECTION OF IgGANTI-IDIOTYPE DIRECTED AGAINST ANTI-HBS MOLECULES
He Dongfeng Nanchong prefecture Women and children Health Centre. A SOLID PHASE LEVERSE PASSIVE HEMAGGLUTINATI-ON FOR THE DETECTION OF IgGANTI-IDIOTYPE DIRECTED AGAINST ANTI-HBS MOLECULES[J]. Journal of North Sichuan Medical College, 1991, 0(2)
Authors:He Dongfeng Nanchong prefecture Women and children Health Centre
Affiliation:He Dongfeng Nanchong prefecture Women and children Health Centre
Abstract:
This paper introduced a method named solid phase reverse passive hemaggluationthe method used polystryrene microtitre plate(v)covered by goat anti-lgG toabsorb antild antibodies agocinst anit-HBs in the serum which was indicated byaggregation of anti-HBs-labelled SRBe(sheeps RBC)using the positive rate was23.7% for HBsAg-positive sere(n=102)mean while no positive result had been foundfor seren(n=116)from heatlhyepesons.lt is one of simple and convinent methodsneeds no atceptional equipment
Keywords:Solid phase Reverse Passive Hemagglutination  Anti-ld antibody Anti-HBs  
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