心肌梗死患者血管紧张素原基因多态性临床检测意义的研究

目的 探讨血管紧张素原基因（AGT）在人群中的分布、临床常见生化指标与心肌梗死的关系。方法 选取患者组114例及对照组204例，用聚合酶链反应（PCR）、限制性片段长度多态性（RFLP）分析，对群体患者和群体健康对照组进行基因分型，统计分析。结果 ACT基因型频率及等位基因频率分布比较均匀；心肌梗死发生与性别、年龄无特异相关性。174MM型和M174等位基因的频率患者组显著高于健康对照组，携带突变型MM基因型的个体比野生型TT者患心肌梗死的危险性高5．667倍，基因突变与心肌梗死发生相关。其他因素中只有吸烟与否其P值〈0．001。校正主要危险因素后，MM基因型仍与心肌梗死发病显著相关，调整后P值分别为0．043和0．001。结论 AGT基因基因型及等位基因在群体中的分布无年龄、性别差异，MM基因型与心肌梗死有关。

Clinical Study of Polymorphism of Angiotensinogen in patients with Myocardial Infarction.

Objective To study the distribution of angiotensinogen(AGT),the relationship between clinical biochemical indexes and myocardial infarc(MI).Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were applied to analyze the genotype of 114 cases of myocardial infarction and 204 cases as control group.Results The frequency of AGT gene and that of the allele were distributed comparatively equally among people of different sex and ages.The frequency of AGT 174MM and the allele of M174 were significantly higher in MI patients than those in healthy control group.The MI risks of individuals carrying mutational MM gene were 5.667 times those of TT gene of natural type,with statistical significance,showing that there was relation between gene mutation and the occurrence of MI.and the analysis of MI patients and the control group.Among all the factors,the numerical value of smoke indicated P<0.001.After emendation of the major risk factors,MM gene type was still significantly related to the occurrence of MI,with the post adjustment P values being 0.043 and 0.001 respectively.Conclusion There is no age and sexdifference between AGT gene type and the allele among the group of people. MM gene of AGT gene is related to myocardial infarction.