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鲨鱼软骨制剂通过下调Bcl-2诱导K562细胞凋亡
引用本文:王成艳,买霞,陈莉. 鲨鱼软骨制剂通过下调Bcl-2诱导K562细胞凋亡[J]. 解剖科学进展, 2009, 15(2)
作者姓名:王成艳  买霞  陈莉
作者单位:1. 武警医学院,训练部教保处,天津,300162
2. 武警医学院,细胞生物学教研室,天津,300162
基金项目:武警部队后勤部基金资助项目 
摘    要:目的探讨鲨鱼软骨制剂(SCP)诱导人红白血病细胞系(K562)凋亡的作用机制。方法以不同浓度SCP加入体外培养的K562细胞中,用MTT比色法检测细胞存活率;Hoechst33342/PI荧光染色,荧光显微镜分析凋亡细胞百分率;流式细胞术进行细胞凋亡定量;免疫细胞化学染色法检测Bcl-2蛋白的表达。结果SCP明显抑制K562细胞生长,IC50值为1mg/ml以下;荧光显微镜下可见50%以上细胞为凋亡细胞的形态学改变;免疫细胞化学检测显示SCP诱导细胞凋亡过程中Bcl-2表达明显降低。结论SCP诱导K562细胞凋亡,可能与下调Bcl-2表达有关。

关 键 词:鲨鱼软骨制剂  K562细胞  细胞凋亡  Bcl-2

Shark Cartilage Preparation Induces Apoptosis of K562 Cells by Downregulation of Bcl-2 Expression
WANG Cheng-yan,Mai Xia,CHEN Li. Shark Cartilage Preparation Induces Apoptosis of K562 Cells by Downregulation of Bcl-2 Expression[J]. Progress of Anatomical Sciences, 2009, 15(2)
Authors:WANG Cheng-yan  Mai Xia  CHEN Li
Affiliation:1.Department of Teaching and Safeguard;2.Department of Cellular Biology;Medical College of the Chinese People's Armed Police Forces;Tianjin 300162 China
Abstract:Objective To investigate the effects of Shark Cartilage Preparation(SCP)on K562 cells.Methods Different concentrations of SCP were added into the cultured cells,cell survival rate was analyzed by MTT assay and the apoptosis of K562 was detected by Hoechst33342/PI and flow cytometer(FCM)for quantitative analysis of apoptosis.The level of Bcl-2 protein was measured with immunocytochemistry.Results The growth of K562 cells was remarkably inhibited by SCP,the IC50 values of SCP for K562 cells were found to be ...
Keywords:Bcl-2
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