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| 实验性肝纤维化形成和逆转过程中基质金属蛋白酶-13蛋白及其mRNA表达 | |
| 引用本文： | 赵志海,辛绍杰,赵景民,王松山,刘萍,尹铁勇. 实验性肝纤维化形成和逆转过程中基质金属蛋白酶-13蛋白及其mRNA表达[J]. 军医进修学院学报, 2002, 23(3): 214-216 |
| 作者姓名： | 赵志海 辛绍杰 赵景民 王松山 刘萍 尹铁勇 |
| 作者单位： | 1. 解放军总医院,99级硕士研究生,北京,100853 2. 解放军302医院,二科,北京,100039 3. 解放军302医院,病理科 |
| 基金项目： | “九五”军队科研基金项目 ( 1998M 152 ) |
| 摘 要： | 目的：研究基质金属蛋白酶－13（matrix metalloproteinase 13,MMP-13)在大鼠四氯化碳实验性肝纤维化形成和逆转过程中的表达。方法：应用免疫组织化学和核酸原位杂交方法研究MMP－13蛋白及其mRNA在四氯化碳实验性大鼠肝纤维化形成和逆转过程中的表达变化，并进行网织纤维染色和胶原纤维染色。结果：MMP－13蛋白及其mRNA在肝脏间质细胞和部分肝细胞中表达。在纤维化形成过程中MMP－13蛋白及其mRNA的表达量相对增加；在纤维化逆转过程中MMP－13蛋白及其mRNA的表达量是由强渐弱的动态变化。结论：肝脏间质细胞是MMP－13蛋白及其mRNA的主要细胞来源，MMP－13在肝纤维化形成和逆转过程中具有重要作用。 |
| 关 键 词： | 肝纤维化 形成 逆转 mRNA 基质金属蛋白酶 实验研究 |
Expression of matrix metalloproteinase13 and its mRNA in carbon-tetrachoride-injured liver fibrosis and its reversal |
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| ZHAO Zhi hai,XIN Shao jie,ZHAO Jing min,Wang Song shan,Liu Ping,Yin Tie yong. Expression of matrix metalloproteinase13 and its mRNA in carbon-tetrachoride-injured liver fibrosis and its reversal[J]. Academic Journal of Pla Postgraduate Medical School, 2002, 23(3): 214-216 | |
| Authors: | ZHAO Zhi hai XIN Shao jie ZHAO Jing min Wang Song shan Liu Ping Yin Tie yong |
| Abstract: | Objective:To explore the expression of matrix metalloproteinase13(MMP 13) and its mRNA in carbon tetrachoride injured liver fibrosis and its reversal Methods:By immunohistochemical analysis and nucleic acid in situ hybridization(ISH) we observed the expression of MMP 13 and its mRNA in specimens from liver of Wistar rats induced by carbon tetrachloride Results:Expression of MMP13 and its mRNA was found mainly in mesenchymal cells, also in parts of hepatocytes The levels of MMP 13 expression were increased in the early time of liver fibrosis and decreased gradualy in the reversal of liver fibrosis Conclusions:Mesenchymal cell is the main cellular origin of MMP13 and its mRNA MMP13 may play an important role in the liver fibrogenesis and its reversal |
| Keywords: | liver cirrhosis matrix metalloproteinases RNA messenger |
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