吉西他滨对慢性粒细胞白血病患者骨髓细胞G-CSFR及bcr/abl mRNA的影响

为了观察吉西他滨对慢性粒细胞白血病(CML)患者骨髓粒细胞集落刺激因子受体(G-CSFR)及bcr/ablmRNA的作用,收集CML慢性期、急变期患者的及骨髓象正常的非血液病患者骨髓液,分为吉西他滨组(加入吉西他滨,调整浓度为10μg/ml)和对照组,培养48小时后收集细胞。采用流式细胞术检测各组标本骨髓G-CSFR的表达率;采用RT-PCR技术检测各组标本bcr/abl mRNA的表达。结果表明:吉西他滨组CML慢性期、急变期患者及骨髓象正常者骨髓G-CSFR的表达率分别为(50.72±8.57)%、(36.32±4.25)%和(59.42±7.62)%。对照组CML慢性期、急变期患者及骨髓象正常者骨髓粒系G-CSFR的表达率分别为(45.42±6.52)%、(30.58±5.68)%和(58.56±5.54)%。两组CML患者骨髓G-CSFR的表达率分别与骨髓象正常组比较,差异有显著性(p0.05),CML慢性期患者与急变期比较,差异有显著性(p0.05)。吉西他滨组CML患者G-CSFR的表达率与未加药组相应组别比较,差异有显著性(p0.05)。吉西他滨组CML慢性期、急变期患者bcr/abl mRNA表达水平比较,差异无显著性(p0.05)。吉西他滨组与对照组、CML慢性期、急变期患者G-CSFR表达率与bcr/abl mRNA表达水平呈负相关(p0.05)。结论:在体外培养下,吉西他滨可以提高CML慢性期、急变期患者骨髓G-CSFR的表达率,对bcr/abl mRNA表达的作用不明显,CML慢性期、急变期患者G-CSFR表达率与bcr/abl mRNA表达水平呈负相关。

Effect of Gemcitabine on Granulocyte Colony-Stimulating Factor Receptor and bcr/abl mRNA in Patients with Chronic Myeloid Leukemia

This study was purposed to investigate the effect of gemcitabine（GEM） on granulocyte colony-stimulating factor receptor（G-CSFR） and bcr/abl mRNA in patients with chronic myeloid leukemia（CML）.23 cases of CML in chronic phase,8 cases of CML in blastic phase and 10 cases of non-hematologic diseases with normal bone marrow were enrolled in this study.The bone marrow from all these cases was collected and divided into 2 group： GEM group（bone marrow cells of CML patients and normal bone marrow cells were cultured with 10 μg/ml GEM for 48 hours） and control group（above-mentioned bone marrow cells were cultured without GEM for 48 hours）.The expression of G-CSFR was detected by flow cytometry,the expression of bcr/abl mRNA was assayed by RT-PCR.The results showed that the G-CSFR expression rates of bone marrow in CML chronic phase and blastic phase as well as normal bone marrow in GEM group were（50.72 ±8.57）%,（36.32±4.25）% and（59.42±7.62）% respectively,while the G-CSFR expression rates of above-mentioned bone marrow in control group were（45.42±6.52）%,（30.58±5.68）% and（58.56±5.54）% respectively.The comparison of G-CSFR expression rates between bone marrow of CML and normal bone marrow,between bone marrow of chronic phase and blastic phase and between bone marrow of GEM group and control group all demonstrated significant difference（p0.05）.The RT-PCR assay showed that the expressions of bcr/abl mRNA in CML chronic and blastic phases of GEM group were（0.59±0.15）% and（0.60±0.13）% respectively,while above-mentioned indicators of control group were（0.60±0.10）% and（0.63±0.11）%;there was no significant difference on expression of bcr/abl mRNA between GEM and control groups（p0.05）.The negative correlation of G-CSFR expression rate with bcr/abl mRNA expression level was observed in GEM and control groups as well as in CML chronic phase and blastic phase of GEM group（r=-0.747,p0.01;r=-0.803,p0.01 respectively）.It is concluded that the GEM can in vitro enhance the expression rate of bone marrow G-CSFR in CML patients at chronic or blastic phases,but no significant effect on expression of bcr/abl mRNA.The negative correlation of G-CSFR expression rate with bcr/abl mRNA expression level exists in CML patients at chronic or blastic phases.