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| BTG1在肾透明细胞癌组织中的表达及对786-O细胞株增殖和凋亡的影响 | |
| 引用本文： | 曹倪豪,陈明. BTG1在肾透明细胞癌组织中的表达及对786-O细胞株增殖和凋亡的影响[J]. 东南大学学报(医学版), 2011, 30(4): 583-587. DOI: 10.3969/j.issn.1671-6264.2011.04.014 |
| 作者姓名： | 曹倪豪 陈明 |
| 作者单位： | 1. 东南大学,医学院,江苏,南京,210009 2. 东南大学,附属中大医院,江苏,南京,210009 |
| 摘 要： | 目的:探讨B细胞易位基因1(BTG1)在肾透明细胞癌组织中的表达及对肾透明细胞癌细胞株786-O增殖和凋亡的影响.方法:采用免疫组化法检测20例肾透明细胞癌及其癌旁组织中BTG1蛋白的表达.构建BTG1表达质粒及合成BTG1的干扰RNA分别转染786-O细胞,通过MTT法实验观察BTG1对786-O细胞体外增殖的影响,... |
| 关 键 词： | 肾透明细胞癌 B细胞易位基因1 抑癌基因 增殖 凋亡 |
Expression of BTG1 in renal clear cell carcinoma and its role in 786-O cell proliferation and apoptosis in vitro |
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| CAO Ni-hao,CHEN Ming. Expression of BTG1 in renal clear cell carcinoma and its role in 786-O cell proliferation and apoptosis in vitro[J]. Journal of Southeast Univ: Medical Sci Ed, 2011, 30(4): 583-587. DOI: 10.3969/j.issn.1671-6264.2011.04.014 | |
| Authors: | CAO Ni-hao CHEN Ming |
| Affiliation: | CAO Ni-hao1,CHEN Ming2(1.School of Medicine,Southeast University,Nanjing 210009,China,2.Zhongda Hospital,China) |
| Abstract: | Objective: To explore the expression of BTG1 in renal clear cell carcinoma(RCCC) and its role in 786-O cells proliferation and apoptosis in vitro.Methods:The expression of BTG1 in 20 RCCC specimens was examined using immunohistochemical staining,the BTG1 expression plasmid was constructed and BTG1 siRNA was designed,followed by respective transfer into 786-O cells.Observation on its role in cell proliferation and apoptosis was performed with MTT and Hoechst respectively.Results:Immunohistochemistry demonstr... |
| Keywords: | renal cell carcinoma B-cell translocation gene 1 tumor suppressor gene proliferation apoptosis |
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