温阳活血方及丹参对硬皮病小鼠模型的影响

目的 研究温阳活血方及丹参对硬皮病小鼠模型的影响。方法 将温阳活血方及丹参应用于博来霉素诱导的硬皮病小鼠模型。用药小鼠分成早期给药组和硬化后给药组。两组分别分为口饲温阳活血方组、口饲丹参组和静脉注射丹参组。于用药后不同时期进行皮肤、肺组织病理学及血清自身抗体检测分析。结果 与未用药的同期对照组相比,硬化后给药组至给药第5周末皮肤组织学无明显改善。早期给药组给药第5周末亦无组织学上的明显改善;第8周末,皮肤厚度比同期对照组有所降低,胶原纤维增生则有较明显改善,尤其是口饲温阳活血方组和静脉注射丹参组(P<0.05)。肺组织学和血清自身抗体检测提示,硬化后给药和早期给药组均无明显改善。结论 早期给药组小鼠随用药时间的递增,皮肤硬化得到明显改善,其中义以口饲温阳活血方组、静脉注射丹参组改善效果更明显;硬化后给药组小鼠皮肤硬化改善不明显。各组小鼠肺纤维化和血清自身抗体均无明显改善。

Effects of Traditional Chinese Herbs, Wen Yang Huo Xue Decoction and Salvia Miltiorrhiza, on Animal Model of Sclerotic Skin

Objectives To study the effects of traditional Chinese herbs, Wen Yang Huo Xue Decoction (herbs of removing blood-stasis and warming the kidney-yang) and Salvia miltiorrhiza, on the animal model of sclerotic skin. Methods A mouse model for sclerotic skin was established in C3H/He mice by repeated local injections of bleomycin for 3 weeks. Wen Yang Huo Xue Decoction was given orally, and Salvia miltiorrhiza orally or intravenously, to the mouse model. The administrations started either simultaneously or after 3 weeks' injections of bleomycin. Mouse skins and lungs were examined histopathogically, and sera were tested for autoantibodies. Results The administrations of herbs, started either at the beginning or at the time sclerosis was induced, caused no significant alleviation of dermal sclerosis by the end of 5 weeks' treatment. After 8 weeks' administrations of herbs, the dermal thickness reduced and collagen histochemical index decreased significantly, especially in group Wen Yang Huo Xue Decoction was given orally and in group Salvia miltiorrhiza was given intravenously at early stage (P< 0.05). Both groups showed no significant improvement on lung flbrosis and serum autoantibodies. Conclusions Significant reduction of dermal sclerosis could be observed by orally taken Wen Yang Huo Xue Decoction and intravenously injected Salvia miltiorrhiza at the early stage and over a long time, but no significant improvement on lung flbrosis or serum antibodies was observed in mouse model of sclerotic skin.