恶性细胞中巨噬细胞集落刺激因子及其受体的异源表达

业已证明血清M-CSF(可溶性M-CSF)水平在白血病前期、白血病、淋巴瘤、乳腺癌和肝癌患者明显升高,但机制不明。为探讨细胞内M-CSF及其受体表达在病态造血,肿瘤和白血病中的作用,采用RT-PCR和ABC免疫酶标技术,对12名正常人静脉血,21例初诊血液病患者骨髓和6株血源细胞系的M-CSF/M-CSF-R mRNA及抗原表达进行了分析。结果表明M-CSF及其受体的表达呈多态性分布,无论是因子还是受体在白血病患者的粒、单、红、巨核系的细胞膜、质、核呈不同程度的反应,提示其在不同状态和病种作用中的复杂性,值得深入研究。

Heterogenic expression of macrophage colony-stimvlatiag factor and its receptor in malignant hematopoietic cells

Macrophage colony-stimulating factor(M-CSF), or named colony-stimulating factor-l(CSF-l), is the cy-tokine required for proliferation, differentiation and survival of monocyte-macrophage lineage and secreted by a variety of cells, such as monocyte-macrophages, lymphocytes, stroma cells, endothelial cells etc. It has been demonstrated that the circulating levels of M-CSF in patients with preleukemia, leukemia, lymphoma, breast cancer and acute/chronic liver diseases etc were significantly increased, but there was no correlation with count of blood monocytes. The mechanism underlying these phenomina is unclear. In this report heterogenic expression of M-CSF/M-CSF-R was studied with ABC-immunoperoxidase assay and RT-PCR assay on 12 donors, 21 patients, and 6 cell lines to search the possibility of heterogenic source of M-CSF in human serum. The results showed that the expression of M-CSF and its receptor were polymorphic: both the M-CSF and its receptor were detected on cell membrane, cytoplasm and nucleus in different lineage of patients. This result suggested the complexity of the expression of M-CSF/M-CSF-R in various conditions and diseases. Meanwhile, the high level of M-CSF/M-CSF-R expression might relate to some abnormal hematopoiesis and leukemogenesis especially in some types of myeloid leukemia.