The role of components of the extracellular matrix and inflammation on oral squamous cell carcinoma metastasis |
| |
Authors: | Tuncay Tanis Zeynep Birsu Cincin Bilge Gokcen-Rohlig Elif Sinem Bireller Murat Ulusan Cem Rustu Tanyel Bedia Cakmakoglu |
| |
Institution: | 1. Department of Otorhinolaryngology, Faculty of Medicine, Istanbul University, Istanbul, Turkey;2. Department of Molecular Medicine, Institute of Experimental Medical Research, Istanbul University, Istanbul, Turkey;3. Department of Maxillofacial Prosthodontics, Faculty of Dentistry, Istanbul University, Istanbul, Turkey |
| |
Abstract: | ObjectivesOral squamous cell carcinoma (OSCC) accounts for about 90% of malignant oral lesions, and is identified as the most frequently occurring malignant tumour of oral structures. We aimed to investigate the genes and pathways related with metastasis on Turkish OSCC patients.Materials and methodsWe performed whole genome expression profiling array on an Illumina platform. A total of 24 samples with 12 OSCC and 12-paired controls that had no tumour were included in the study. Hierarchic clustering and heat map were used for data visualisation and p-values assessed to identify differentially expressed genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Ingenuity Pathway Systems (IPA) analysis were performed to consider biologic meaning of differential expression of the genes between tumour and control groups.ResultsWe identified 790 probe sets, corresponding to 648 genes that were effective in separating invasive and metastatic OSCC. Consequently, we found statistically relevant expression results on extracellular matrix members on MMPs such as MMP3, MMP10, MMP1 and MMP9; on laminin such as LAMC2, LAMA3 and LAMB3; several genes in the collagen family; and also on chemokines from the inflammation process.ConclusionStatistically relevant expression changes for MMPs, laminins, collagens, and chemokines, which are components of the extracellular matrix and inflammation process, may be considered as a molecular biomarker for early prediction. Further studies are necessary to determine and understand the molecular mechanisms that underlie OSCC metastasis. |
| |
Keywords: | OSCC Microarray Metastasis |
本文献已被 ScienceDirect 等数据库收录! |
|