女贞子总黄酮对HepG2细胞脂代谢调节作用的研究

Objective To study molecular target of total flavonoid in Ligustrum Lucidum Ait(TFL) on lipometabolism regulation of HepG2 cell.Methods Treatment doses of total flavonoid in Ligustrum Lucidum Ait were determined by MTT and LDH.The expression of lipoprotein lipase(LPL),3-hydroxy-3-methylglutary-coenzyme A redutasein(HMGCR)and peroxisome proliferator-actived receptorsα(PPARα)mRNA of HepG2 cell were determined by reversed transcription polymerase chain reaction(RT-PCR).Results Increased expression of LPL mRNA and PPARαmRNA with TFL(10-6～10-4g/ml)were found in HepG2 cells after treated with TFL(10-5g/ml),but decreased expression of HMGCR mRNA was found after treated with TFL(10-5,10-4 g/ml).Conclusion The potential mechanisms of total flavonoid in Ligustrum Lucidum Ait's hypolipidmic effects may be with its functions of regulating lipoprotein degradation through PPARα-LPL pathway,or regulating steroid biosynthesis by reducing HMGCR.

Study of total flavonoid in Ligustrum Lucidum Ait on lipometabolism regulation of HepG2 cell