膀胱移行细胞癌患者外周血CK20表达的研究

目的：探讨CK20在膀胱移行细胞癌(TCCB)患者外周血的表达及其意义。方法：采用逆转录聚合酶链反应(RTPCR)检测63例TCCB患者和19例健康志愿者以及19例非肿瘤患者外周血CK20表达。以CAPDH作为内参照。结果：19例健康志愿者和19例非肿瘤患者外周血CK20的表达均为阴性；63例TCCB患者外周血CK20阳性表达率为25．4％(16／63)，其中T1期为0(0／31)，T2期为12．5％(2／16)，T3期为77．8％(7／9)，T4期为100％(7／7)，CK20阳性表达率随分期增加而增加，T3～4期明显高于T1期和T2期。对T1～3期33例患者随访6个月，其中外周血CK20阴性表达26例未发现远处转移；外周血CK20阳性表达7例中2例发生远处转移。结论：检测外周血CK20表达，可以提示癌细胞的血行播散，对判断预后以及指导治疗具有一定临床价值。

Detection of CK20 in peripheral blood of patients with transitional cell carcinoma of the bladder

Objective:To study the expression of cytokeratin (CK) 20 in the peripheral blood of patients with transitional cell carcinoma of the bladder and to evaluate its clinical significance.Methods:CK20 mRNA in 63 patients with TCCB,19 non-cancerous patients and 19 healthy volunteers was detected respectively.GAPDH was used as a general marker for normal blood cells.CK20 and GAPDH were amplified by using RT-PCR.Results:Nineteen healthy volunteers and 19 non-cancerous patients showed no CK20 signals in peripheral blood.Sixteen of 63(25.4%) patients with TCCB showed positive CK20 signals in peripheral blood,including 0/31 of stage T 1, 2/16 of stage T 2, 7/9 of stage T 3,7/7 of stage T 4.The CK20 expression difference between T 1/T 2 group and T 3-4 groups was significant.Two patients with positive CK20 signals in peripheral blood had distant metastases during a 6-month follow-up period.Conclusion:CK20 is applicable to detect cancer cells in the peripheral blood of transitional cell carcinoma of the bladder and may play an important role in the evaluation of the prognosis and postoperative management of TCCB.