柯萨奇病毒-腺病毒受体在结肠癌组织中的表达及意义

目的：柯萨奇病毒-腺病毒受体（coxsackie and adenovirus receptor，CAR）介导腺病毒与靶细胞之间的粘附，是腺病毒转染靶细胞的限速因子。本文分析CAR在瘤旁上皮组织、结肠肿瘤原发灶和淋巴结转移灶中的表达．为设计腺病毒载体基因治疗方案提供依据。方法：采用免疫组织化学方法检测62例结肠癌组织中CAR的表达．分析CAR表达与临床病理特征的关系。结果：与瘤旁上皮组织相比，肿瘤组织普遍存在CAR表达下调．但CAR在原发灶和淋巴结转移灶的表达水平无明显差异。CAR的表达水平与患者年龄及肿瘤大小相关。结论：结肠癌组织中存在CAR表达下调，提示在设计腺病毒载体基因治疗方案时应充分考虑CAR表达变化对疗效的影响.

Expression of Coxsackie and Adenovirus Receptor and its Significance in Human Colon Carcinoma

Objective: To optimize adenovirus vector-based gene therapy and to analyze the expression of the coxsackie and adenovirus receptor (CAR) in normal colon tissues, primary tumors and lymph node metastasis of colon carcinoma. Methods: The expression of CAR in 62 cases of colon carcinoma was detected using immunohistochemistry and the relationship between the expression of CAR and clinicopathologic characteristics was analyzed. Results: Compared with paraneoplastic epithelial tissue of the colon, the expression of CAR was generally downregulated in tumor tissues, whereas for the cases with lymph node metastasis, the expression level of CAR in primary tumor and lymph node metastasis was identical. The expression of CAR was associated with patient age and tumor size. Conclusion: Downregulated expression of CAR is commonly seen in colon carcinoma, suggesting that the influence of CAR expression on therapeutic efficacy should be carefully considered when planning a regimen of gene therapy using adenovirus vector.