8 103钯胆管放射性支架释放的γ射线诱导胆管癌细胞凋亡的研究

目的：探讨103钯（103pd）释放的γ射线治疗胆管癌的作用机制。方法：用MTT法分析103pd释放的γ射线对胆管癌细胞生长的影响，用电镜、DNA凝胶电泳和流式细胞仪观察细胞形态和凋亡率的变化。结果：103pd释放的γ射线明显抑制胆管癌细胞生长。5.333mci剂量的γ射线诱导出典型的细胞凋亡特征，如凋亡小体、特征性亚G1峰等。6.645mci以上剂量的γ射线导致细胞坏死。结论：γ射线诱导胆管癌细胞凋亡可能是放射治疗胆管癌的重要机制之一，103pd释放的γ射线诱导胆管癌细胞凋亡与照射剂量有密切关系。

Study on induction of apoptosis of biliary duct cancer cells by γ-ray of the 103pd biliary stent

Abstract:Objective：To investigate the mechanism and role of γ-ray of 103pd in the treatment of biliary duct cancer.Methods ：A series of biliary duct cancer cells were treated with different γ-ray dose, and MTT ［3-(4,5-dimethy thiazol-2-yl)-2,5-diphenyl terazolium-bromide］ technique was used to determine the inhibition rate of γ-ray of 103pd on the biliary duct cancer cells; and electron micro-technique, DNA agarose gel electrophoresis and flow cytometry to evaluate the morphological characteristics and apoptosis rate of the biliary duct cancer cells were also used.Results：The γ-ray radiation of 103pd resulted in significant inhibition of the biliary duct cancer cells. The features of biliary duct cancer cells apoptosis(e,g：apoptic bodies, DNA ladders band hypodiploid DNA peak) could be seen in the group with lower dosage (5.333mci), and cell necrosis was seen in higher dosage (more than 6.645 mci).Conclusions：The γ-ray radiation could induce apoptosis of the biliary duct cancer cells, but with dose dependence, and apoptosis can be an important mechanism for radiation treatment of biliary duct cancer.