白芍总苷对糖尿病大鼠心肌氧化应激和细胞凋亡的影响及机制研究

目的 探讨白芍总苷(total glucosides ofpaeony,TGP)对糖尿病大鼠心肌氧化应激和细胞凋亡的影响及其机制.方法 取120只SD大鼠按随机数字表法分为对照组、模型组、二甲双胍(Met,200 mg/kg)组和TGP低、中、高剂量(50、100、200 mg/kg)组,每组20只;采用一次性ip链脲...

Effects of total glucosides of paeony on myocardial oxidative stress and apoptosis in diabetic rats

Objective To investigate the effect of total glucosides of paeony(TGP) on myocardial oxidative stress and apoptosis in diabetic rats and its mechanism. Methods Tatolly 120 SD rats were randomly divided into control group, model group, metformin group(70 mg/kg) and TGP low-, medium-and high-dose groups(50, 100 and 200 mg/kg). The diabetic rat models were prepared by ip injection of streptozotocin(65 mg/kg). After the model was established, the drugs were given by gavage simultaneously, once a day for eight weeks, while the rats in the control group and model group were given normal saline. The fasting blood glucose level was detected by blood glucose meter, the content of LDH, CK-MB, ALT in serum and the content of MDA, the activity of ntioxidant enzyme(SOD, GSH-Px) in myocardial were etected by biochemical analysis;The pathological changes and apoptosis of myocardium were observed by HE staining and TUNEL staining;The expression of GRP78, CHOP, Cleved Caspase-12 protein in myocardial tissue was detected by Western blotting method. Results Compared with model group, the fasting blood glucose levels of rats in metformin group and TGP medium-, high-dose groups were significantly decreased;the content of LDH, CK-MB, ALT in serum and MDA in myocardial were significantly decreased, and the activity of SOD, GSH-Px in myocardial were significantly increased;the pathological damage and apoptosis of myocardial tissue were significantly improved, the AI was significantly decreased;the expression of GRP78, CHOP, Cleved Caspase-12 were significantly down-regulated;the differences were significant(P < 0.05 or 0.01). Conclusion TGP has inhibitory effect on myocardial oxidative stress and apoptosis in diabetic rats, and its mechanism may be related to increasing antioxidant enzyme activity and inhibiting endoplasmic reticulum stress apoptosis pathway.