Copper-64-diacetyl-bis (N4-methylthiosemicarbazone) accumulates in rich regions of CD133+ highly tumorigenic cells in mouse colon carcinoma

Introduction⁶⁴Cu-diacetyl-bis (N⁴-methylthiosemicarbazone) (⁶⁴Cu-ATSM) is a potential imaging agent of hypoxic tumor for use with PET. Recent literature demonstrated that cancer cells expressing CD133, which is a frequently used marker for so-called cancer stem cells or cancer stem cell-like cells (collectively referred to here as CSCs), contribute to tumor's therapeutic resistance and metastasis ability. Culturing under hypoxia is also reported to enlarge the proportion of CD133⁺ cells, which would indicate survival advantage of CD133⁺ cells under hypoxia. Here, we investigated the relationships between ⁶⁴Cu-ATSM accumulation and existence of CD133⁺ cells using mouse colon carcinoma (colon-26) tumor.MethodsIntratumor distribution of ⁶⁴Cu-ATSM and ¹⁸F-fluorodeoxyglucose (¹⁸FDG) was compared with immunohistochemical staining for CD133 with a colon-26 model. In vitro characterization of CD133⁺ colon-26 cells was also performed.ResultsIn colon-26 tumors, ⁶⁴Cu-ATSM localized preferentially in regions with a high density of CD133⁺ cells. The percentage of CD133⁺ cells was 11-fold higher in ⁶⁴Cu-ATSM high-uptake regions compared with ¹⁸FDG high- (but ⁶⁴Cu-ATSM low-) uptake regions. CD133⁺ colon-26 cells showed characteristics previously linked with CSCs in other cancer cell lines, such as high colony-forming ability, high tumor-initiating ability and enrichment under hypoxic cultivation. The proportion of CD133⁺ cells was enlarged by culturing under glucose starvation as well as hypoxia, and ⁶⁴Cu-ATSM uptake was increased under such conditions.ConclusionsOur findings showed that, in colon-26 tumors, ⁶⁴Cu-ATSM accumulates in rich regions of CD133⁺ cells with characteristics of CSCs. Therefore ⁶⁴Cu-ATSM could be a potential imaging agent for rich regions of CD133⁺ cells, associated with CSCs, within tumors.