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高效液相色谱用于布鲁氏菌标准株的鉴别试验
引用本文:卿燕,刘志文,严蕾,张松. 高效液相色谱用于布鲁氏菌标准株的鉴别试验[J]. 地方病通报, 1986, 0(3)
作者姓名:卿燕  刘志文  严蕾  张松
作者单位:新疆地方病防治研究所 乌鲁木齐(卿燕,刘志文,严蕾),新疆地方病防治研究所 乌鲁木齐(张松)
摘    要:用高效液相色谱法对羊种、牛种、猪种、犬种、鼠种和绵羊副睾种布鲁氏菌6个标准菌种菌株进行鉴别试验,以目视法观察菌株核酸样品的峰形数量、高度以及出峰保留时间,分析各菌株图谱的A区,结果表明:6个布鲁氏菌种都出现了各自独特的洗脱图峰,峰形清晰可辨,并且B区和C区峰形可能是布鲁氏菌属的“特征峰区”。

关 键 词:高效液相色谱法  布鲁氏菌核酸  菌种鉴别试验

THE USE OF HIGHLY EFFECTIVE LIQUID CHRO-MATOGRAPHY IN THE IDENTIFICATION OF STANDARD STRAINS OF BRUCELLA
Qing Yan et al. THE USE OF HIGHLY EFFECTIVE LIQUID CHRO-MATOGRAPHY IN THE IDENTIFICATION OF STANDARD STRAINS OF BRUCELLA[J]. Endemic Diseases Bulletin, 1986, 0(3)
Authors:Qing Yan et al
Abstract:Six standard strains of B. melitensis, B. abortus, B. suis, B. canis, B. neoto-mae and B. ovis underwent identification by highly effective liquid chromatograp-hy. The number of peak patterns, their height and the peak time of samples of nucleic acid of the strains were studied and the region A in the figures of these strains was analysed. The results showed that all the 6 Brucella strains had their distinct elution peak patterns and that the peak forms in region B and C might be the "characteristic peak region" for Brucella.
Keywords:highly effective liquid chromatography-Brucella nucleic acid-bac-terial species identification  
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