复方戊二醛消毒液杀菌效果影响因素的研究

目的研究复方戊二醛消毒液杀菌效果的影响因素。方法采用悬液定量杀菌试验方法,对温度、pH值、有机物等影响因素进行了实验室观察。结果用含量1 9200 mg/L戊二醛消毒液原液对悬液内枯草杆菌黑色变种芽孢作用2 h,平均杀灭对数值为5.27。试验温度由30℃降至10℃时,用该消毒液原液对枯草杆菌黑色变种芽孢作用1 h,平均杀灭对数值由4.23降至3.26。将该消毒液原液pH值由4.0调节到8.0,作用3 h对枯草杆菌黑色变种芽孢的平均杀灭对数值由0.97增加到8.40。菌悬液内含50%的小牛血清对该消毒液原液的杀灭芽孢作用有轻度影响。戊二醛消毒液内加入亚硝酸钠或加入聚氧乙烯脂肪醇醚均可明显增强其杀菌作用。结论复方戊二醛消毒液随温度和pH值升高,其杀菌作用增强;加入亚硝酸钠或聚氧乙烯脂肪醇醚有明显增效作用;有机物对其杀菌作用有一定降低。

STUDY ON FACTORS INFLUENCING GERMICIDAL EFFICACY OF COMPOUND GLUTARALDEHYDE DISINFECTANT

Objective To study the factors influencing germicidal efficacy of compound glutaraldehyde disinfectant.Methods Suspension quantitative germicidal tests were used to observe the influential factors such as temperature,pH value and organic substance.Results The average killing logarithm values of spores of Bacillus subtilis var.niger exposed to the original disinfectant solution containing glutaraldehyde 19200 mg/L for 2 h was 5.27.When the temperature during exposure was reduced from 30℃ to 10℃,the average killing logarithm value of bacterial spores exposed to the original disinfectant solution for 1 h was reduced from 4.23 to 3.26.When the pH value of the original disinfectant solution increased from 4.0 to 8.0,the average killing logarithm value of the bacterial spores exposed to the disinfectant solution for 3 h was increased from 0.97 to 8.40.Adding 50% calf serum to the bacterial suspension slightly influenced the germicidal efficacy of the original disinfectant solution in killing the bacterial spores.Adding sodium nitrite or polyoxyethylene aliphatic alcohol ether to the disinfectant solution could obviously enhance its germicidal efficacy.Conclusion The germicidal efficacy of compound glutaraldehyde disinfectant could be enhanced by increasing temperature and pH value.Adding sodium nitrite or polyoxyethylene aliphatic alcohol ether has obvious synergetic effect.However,the organic substance can reduce the germicidal efficacy.