Galectin-3在大肠癌组织中的表达及其临床意义

目的研究Galectin-3在大肠癌组织、癌旁组织及转移组织中的表达,初步探讨其与大肠癌发生发展,侵袭转移的关系。方法(1)用SP免疫组化法检测158例临床石蜡组织标本(癌旁组织30例,腺瘤组织25例,大肠癌组织65例,淋巴结转移性大肠癌38例)中的Galectin-3蛋白的表达。(2)用RT-PCR法分析Galectin-3mRNA在31对新鲜大肠癌组织及相应癌旁组织中的表达情况。结果(1)Galectin-3蛋白在大肠癌组织、癌旁组织及腺瘤中的表达具有明显差异(P<0.001);在浸润程度深、分化程度低及发生淋巴结转移的大肠癌组织中的表达明显高于浸润程度浅、分化程度高及未发生淋巴结转移的大肠癌组织(P<0.05)。(2)在31对大肠癌组织中浸润程度深、分化程度低及发生淋巴结转移的组织中的表达明显高于浸润程度浅、分化程度高及未发生淋巴结转移的组织,其表达量的平均倍比关系分别为2.67、1.98、1.91。结论Galectin-3的差异表达,可能与大肠癌的发生发展及转移有关。

Association of galectin-3 expression with biological behaviors of human colorectal carcinoma

Objective To investigate the association between galectin-3 expression and biological behaviors of human colorectal carcinoma.Methods SP immunohistochemistry was used to detect the expression of galectin-3 in 158 cases of paraffin-embedded specimens(including 30 normal mucosa,25 adenoma,65 carcinoma and 38 metastatic tumor speciemns).Real-time RT-PCR was performed to detect galectin-3 mRNA expression in 31 pairs of fresh carcinoma and corresponding adjacent normal mucosa specimens.Results The expression levels of galectin-3 was significantly different between normal mucosa and adenoma tissues(P<0.001),and moderately or well differentiated colorectal carcinomas showed significantly lower expression of the galectin-3 than the poorly differentiated carcinomas(P=0.03).Invasive carcinomas exhibited higher galectin-3 expression levels than non-invasive ones(P<0.001),and galectin-3 expression in the colorectal carcinomas was significantly related with the lymph node metastasis(P<0.001).Galectin-3 mRNA expression in poorly differentiated colorectal carcinomas was about 1.98 times that in moderately or well differentiated colorectal carcinomas(P=0.03),and in invasive carcinomas,galectin-3 mRNA expression was 1.67 times higher than that in non-invasive ones(P<0.001).Galectin-3 mRNA expression in tumors with lymph node metastasis was 1.91 times that in non-metastatic tumors(P=0.013).Conclusion Galectin-3 expression is positively correlated with and invasion,poor differentiation and metastasis of colorectal carcinoma.