应用粒-巨噬细胞集落刺激因子治疗小鼠炎症性肠病的疗效研究

目的探讨粒-巨噬细胞集落刺激因子(granulocyte macrophage colony-stimulating factor,GMCSF)对于5%2、4、6-三硝基苯磺酸(TNBS)诱导的结肠炎BALB/c小鼠的治疗作用。方法应用TNBS灌肠建立BALB/c小鼠结肠炎模型。结肠炎小鼠随机分为2组,在造模后3h,分别给予GM-CSF(100μg/kg)或磷酸盐缓冲液(PBS)皮下注射进行治疗干预,连续6日。每日观察小鼠体重,腹泻及便血情况,记录第1,3,5日的疾病活动指数(disease activity index,DAI)评分。造模后第5天处死小鼠,获取结肠组织,根据粘连、溃疡及炎症情况计大体损伤评分。应用H-E染色判定结肠病理组织评分;获取结肠组织匀浆,应用ELISA方法检测炎症细胞因子TNF-α的表达。结果 GM-CSF的治疗明显提高了小鼠的生存率,与PBS对照组相比,治疗组小鼠第3、5日的DAI评分、大体损伤评分及病理组织评分明显降低,GM-CSF组小鼠结肠组织中TNF-α的表达水平下调。结论 GM-CSF治疗减轻了小鼠的结肠炎症。

Granulocyte macrophage colony-stimulating factor ameliorates TNBS-induced murine colitis

Objective To study the therapy effect of granulocyte macrophage colony-stimulating factor（GM- CSF） on Crohn＇s disease induced by 2, 4, 6-trinitrobenzenesulfonic acid solution（TNBS） in murine. Methods BALB/c mice colitis was induced by rectal administration of TNBS, three hours after the administration, BALB/c mice were given GM-CSF（100μg/kg） or phosphate-buffered saline（PBS） subcutaneously once a day from day 0 to day 5. The mice weight, stool, fecal occult blood were observed everyday and DAI （ Disease activity index, DAI ） scores were calculated at day 1, 3, 5. The whole colons were obtained and scored for adhesion, ulcer, and inflammation. Tissue specimens from the descending colon were obtained and stained with HE staining. The expression of pro-inflammatory cytokine proteins TNF-α in colon were further examined by ELISA. Results GM-CSF treatment significantly improved the survival rate of colitis BALB/c mice and downregulated the DAI scores. The general tissue scores and histological scores were less severely, the levels of TNF- α in the colon tissues were significantly lower in the GM-CSF group than in the PBS group （ P 〈0.01 or 0.05）. Conclusion Treatment with GM-CSF ameliorates TNBS-indueed colitis in BALB/c mice.