| icl mRNA特异性10-23DRz在小鼠体内抗结核分枝杆菌感染的实验研究 |
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| 引用本文： | 李俊明,朱道银,王娜,罗清,万腊根. icl mRNA特异性10-23DRz在小鼠体内抗结核分枝杆菌感染的实验研究[J]. 第三军医大学学报, 2010, 32(6): 541-545 |
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| 作者姓名： | 李俊明 朱道银 王娜 罗清 万腊根 |
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| 作者单位： | 南昌大学第一附属医院检验科,南昌,330006;重庆医科大学免疫学教研室,重庆,400016 |
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| 基金项目： | 国家自然科学基金(30270584)~~ |
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| 摘 要： | 目的探讨iclmRNA特异性10-23脱氧核酶(deoxyribozyme,DRz)对结核分枝杆菌(Mycobacterium tuberculosis,Mtb)在小鼠体内感染的影响,及其单独或与异烟肼(isoniazidum,INH)联合应用治疗小鼠结核病的效果。方法分别用DZ4、INH、INH+DZ4-S或INH+DZ4对Mtb进行预处理,再用上述经过预处理的Mtb感染BALB/c小鼠,一定时间后进行小鼠肺组织Mtb培养及病理学改变观察。利用DZ4-FITC溶液对BALB/c小鼠进行滴鼻处理,检测滴鼻途径给药的效果。采用尾静脉注射法建立小鼠结核病模型,将模型小鼠随机分为5组(n=10),分别给予生理盐水、DZ4、INH、INH+DZ4、INH+DZ4-polyG治疗,于治疗完成2周后进行小鼠肺组织Mtb荷菌量检测和病理学改变观察。结果Mtb感染后2周,各组小鼠的肺组织荷菌量均无显著性差异(P>0.05)。感染进行至4～12周时,INH+DZ4预处理组Mtb感染小鼠的肺组织荷菌量较其它各组均显著偏低(P<0.05),肺组织的病理改变较其他各组也明显轻微。经滴鼻途径给予DZ4-FITC溶液4h后,小鼠肺组织冰...
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| 关 键 词： | 分枝杆菌 结核 10-23脱氧核酶 结核病模型 小鼠 |
10-23 deoxyribozyme targeting icl mRNA inhibits Mycobacterium tuberculosis infection in mice |
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| Li Junming,Zhu Daoyin,Wang Na,Luo Qing,Wang Lagen. 10-23 deoxyribozyme targeting icl mRNA inhibits Mycobacterium tuberculosis infection in mice[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(6): 541-545 |
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| Authors: | Li Junming Zhu Daoyin Wang Na Luo Qing Wang Lagen |
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| Affiliation: | 1Department of Clinical Laboratory;First Affiliated Hospital;Nanchang University;Nanchang;Jiangxi Province;330006;2Department of Immunology;College of Basic Medical Sciences;Chongqing Medical University;Chongqing;400016;China |
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| Abstract: | Objective To investigate the effects of 10-23 deoxyribozyme (DRz) targeting the mRNA of isocitrate lyase gene (icl) which play an important role in the persist of Mycobacterium tuberculosis (Mtb) in host macrophage on the infection of the bacterium and the therapeutic effects of 10-23DRz alone or combined with isoniazidum (INH) on mice tuberculosis. Methods According to our previous study, DZ4 (TGAACTGGAAGGCTAGCTACAACGATTGAAGCCC) was identified with the highest cleavage activity among the 10-23 DRz. DZ4-S, ... |
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| Keywords: | Mycobacterium tuberculosis 10-23 deoxyribozyme tuberculosis model mice |
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