两种分离胎盘间充质干细胞方法的比较

目的：寻找从胎盘组织中分离培养间充质干细胞的最佳方法。方法：分别采用外植块贴壁法和酶消化法从胎盘组织中分离间充质干细胞，并对其形态、免疫表型、生长动力检测和多向分化能力进行检测。结果：流式细胞术和诱导分化实验表明酶液消化法分离得到的贴壁细胞表型符合间充质多能干细胞特征，且在合适诱导条件下能向造骨细胞、软骨细胞和脂肪细胞分化，而外植块贴壁法获得的贴壁细胞弱表达CD29和CD105，仅能向脂肪细胞分化。结论：酶消化法可有效的从胎盘组织中分离间充质干细胞，增殖能力强，具备多向分化能力。

Comparison of two methods in the isolation of human placenta mesenchymal stem cells

Objective: To investigate the protocols of isolating mesenchymal stem cells from the placenta and to evaluate their biological characters.Methods: The explant method and enzyme digestion method were used to isolate mesenchymal stem cells from the placenta.Morphology,immunotyping,growth kinetics and mutitopent differentiation of these cells were assayed.Results: FACS and differentiation experiments showed immunotyping of adherent cells isolated by the enzyme digestion method matched with the character of mesenchymal stem cells and could differentiate into osteoblasts,chondrocytes and adipocytes under an appropriate induction condition.Cells isolated by the explant method weakly expressed CD29 and CD105 and could differentiate only into adipocytes.Conclusion: The enzyme digestion method can effectively isolate mesenchymal stem cells with a high proliferative ability and multipotent differentiation ability.