| 中哈边境口岸臀突客蚤检出立克次氏体核酸 |
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| 引用本文： | 罗丹,尹小平,王安东,田延河,梁臻,巴特,张江国. 中哈边境口岸臀突客蚤检出立克次氏体核酸[J]. 中国人兽共患病杂志, 2016, 32(8): 755-759. DOI: 10.3969/j.issn.1002-2694.2016.08.014 |
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| 作者姓名： | 罗丹 尹小平 王安东 田延河 梁臻 巴特 张江国 |
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| 作者单位： | 1.新疆石河子大学医学院,石河子 832000;2.新疆阿拉山口检验检疫局,阿拉山口 833418;3.新疆石河子大学动物科技学院,石河子 832000 |
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| 基金项目： | the National Natural Science Foundation of China(81560338)，Autonomous Region Natural Science Foundation of Xinjiang Uygur (No.2010J01115).国家自然基金(81560338)，新疆维吾尔自治区自然科学基金(201442137-1) |
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| 摘 要： | 目的了解中哈边境地区臀突客蚤(Xenopsylla minax)中立克次氏体携带情况。方法收集阿拉山口口岸臀突客蚤样本,PCR扩增立克次体17 kDa基因片段,PCR产物测序并BLAST比对,利用Mega 6.0构建分子遗传进化树。结果42.86%(3/7)的臀突客蚤携带立克次体,17 kDa基因遗传进化树显示与Candidatus Rickettsia senegalensis和Rickettsia bellii同源性最高。结论中哈边境口岸地区臀突客蚤携带立克次体核酸。
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| 关 键 词： | 立克次体 臀突客蚤 PCR 中哈边境 序列分析 |
| 收稿时间： | 2016-02-14 |
Rickettsia isolated from Xenopsylla minax at China-Kazakh border |
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| LUO Dan,YIN Xiao-ping,WANG An-dong,TIAN Yan-he,LIANG Zhen,BA Te,ZHANG Jiang-guo. Rickettsia isolated from Xenopsylla minax at China-Kazakh border[J]. Chinese Journal of Zoonoses, 2016, 32(8): 755-759. DOI: 10.3969/j.issn.1002-2694.2016.08.014 |
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| Authors: | LUO Dan YIN Xiao-ping WANG An-dong TIAN Yan-he LIANG Zhen BA Te ZHANG Jiang-guo |
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| Affiliation: | 1 College of Medicine,Shihezi University,Shihezi 832000,China;2 Alashankou Entry - Exit Inspection and Quarantine Authority Alashankou 833418,China;3 College of Animal Science and Technology, Shihezi University,Shihezi 832000,China |
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| Abstract: | In order to investigate the infection state of Rickettsia from X. minax at China-Kazakh border,the X. minax were collected,and 17 kDa genes of Rickettsia were tested by PCR,and the sequence was analyed by BLAST and phylogenetic allysied by Mage 6.0. Showed that the positive rate of 17 kDa gene of Rickettsia was 42.86% (3/7) at Alashankou Port. The phylogenetic tree of Rickettsia 17 kDa gene showed that the uncultured Rickettsia sp. was the highest homology with Candidatus Rickettsia senegalensis and R. bellii.In conclusion the X. minax infected Rickettsia at China-Kazakh border. |
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| Keywords: | Rickettsia Xenopsylla minax PCR China-Kazakh border sequence analysis |
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