国产和进口冷冻原肠中奇异变形杆菌的监测与分析

目的研究国产和进口冷冻原肠携带奇异变形杆菌的情况。方法本研究对2014-2016年采集的40份冷冻原肠样品进行检测,其中包括16份国产猪原肠和24份进口羊原肠。经过奇异变形杆菌的分离、表型特征鉴定和16S rDNA鉴定,同时收集GenBank中不同地域和宿主动物的奇异变形杆菌,以及其他重要食源性致病菌的16S rDNA序列,结合本研究测定序列共同进行比对分析。结果40份样品中有10份样品为PM检测阳性,总的PM检测阳性率为25.00%,其中,国产冷冻猪原肠的PM检测阳性率为31.25%,澳大利亚和新西兰进口冷冻羊原肠的PM检测阳性率分别为27.27%和15.38%,另外,发现不同原肠PM分离株之间的16S rDNA同源性差异较大,且同源性的高低和PM菌株的分离地域及宿主动物均未呈现明显的关联性。结论国产和进口冷冻原肠携带奇异变形杆菌的情况比较严重,相关管理和研究应进一步加强。

Monitoring and analysis of Proteus mirabilis in domestic and import frozen intestine

To study the Proteus mirabilis carried by domestic and imported frozen intestines, forty samples collected during 2014-2016, which included sixteen domestic hog intestines and twenty-four imported lamb/sheep intestines, were tested in this study. Based on Proteus mirabilis isolation, phenotype and 16S rDNA identification, and alignment analysis among 16S rDNA sequences obtained in this study, those of Proteus mirabilis isolated in different areas, hosts and other important food-borne pathogenic bacteria available in the GenBank, it showed that ten of forty samples were positive in the test for Proteus mirabilis, and the total positive rate was 25.00%. The positive rate of domestic hog intestines was 31.25%, and that of lamb/sheep intestines imported from Australia and New Zealand was 27.27% and 15.38%, respectively. In addition, the homology of 16S rDNA presented some diversity in Proteus mirabilis strains isolated from intestines, but no significant correlation of evolution between different areas and hosts was found in this study. In conclusion, the situation of the Proteus mirabilis carried by domestic and imported frozen intestines is serious, the relevant management and study should be further strengthened.