大补阴丸治疗2型糖尿病合并骨关节炎的实验研究

目的:观察大补阴丸对2型糖尿病合并骨关节炎大鼠的治疗作用。方法:50只雄性SD大鼠,随机分为5组,每组10只,分别为空白组、模型组、阳性药组、大补阴丸高剂量组、大补阴丸低剂量组,连续给药6周。检测各组大鼠第0周、2周、4周、6周的血糖,骨关节中基质金属蛋白酶-3(MMP-3)含量,关节液中白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)及转化生长因子-β1(TGF-β1)mRNA表达量。结果:大补阴丸可降低实验大鼠血糖,给药组与模型组比较差异具有统计学意义(P<0.01)。大补阴丸可抑制骨关节中异常增多的MMP-3,减少关节液中IL-6、TNF-α及TGF-β1 mRNA表达,给药组与模型组比较差异具有统计学意义(P<0.01,P<0.05)。结论:大补阴丸可通过有效平稳地降低大鼠血糖,减低骨关节中MMP-3含量,减少关节液中IL-6、TNF-α及TGF-β1 mRNA表达量,从而控制血糖抑制炎症反应,发挥治疗2型糖尿病合并骨关节炎的作用。

Experimental Study of Dabuyinwan in Treating Type 2 Diabetes with Osteoarthritis

Objective:To observe the therapeutic effect of Dabuyin Pill on type 2 diabetes mellitus(DM)complicated with osteoarthritis in rats.Methods:50 male SD rats were randomly divided into the blank group,the model group,the positive drug group and the Dabuyin Pill of high-dose and low-dose,with 10 rats in each group.The administration duration was continuous 6 weeks.The blood sugar of rats in each group was measured at 0 w,2 w,4 w and 6 w,the content of MMP-3 in bone and joint was detected,and the expressions of IL-6,TNF-αand TGF-β1 mRNA in synovial liquid were measured.Results:Dabuyin Pill could reduce blood sugar,and the differences between the administration groups and the model group were statistically significant(P<0.01).Dabuyin Pill could inhibit the abnormal increase of MMP-3 in bone and joint,and reduce the expressions of IL-6,TNF-αand TGF-β1 mRNA;of which there were statistical differences between the administration groups and the model group(P<0.01,P<0.05).Conclusion:Dabuyin Pill can effectively and steadily reduce blood sugar,reduce the content of MMP-3 in bone and joint,reduce the expression of IL-6,TNF-αand TGF-β1 mRNA in synovial fluid,thereby controlling blood sugar and inhibiting inflammation to play the role in treating Type 2 DM complicated with osteoarthritis.