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| 白念珠菌CDR1基因上游调控序列与氟康唑耐药的关系初探 | |
| 引用本文： | 贺志彬,廖万清,姚志荣,王志东. 白念珠菌CDR1基因上游调控序列与氟康唑耐药的关系初探[J]. 上海医学, 2005, 28(8): 691-693 |
| 作者姓名： | 贺志彬 廖万清 姚志荣 王志东 |
| 作者单位： | 571159,海南,海口187中心医院皮肤科;第二军医大学附属长征医院皮肤科;上海新华医院皮肤科 |
| 摘 要： | 目的探讨白念珠菌耐药基因CDR1基因上游调控序列对氟康唑耐药的影响。方法分别抽提氟康唑耐药／敏感配对菌株DNA，通过特异性合成CDR1基因上游调控序列引物，分析该序列在氟康唑耐药／敏感配对菌株中是否存在基因突变。结果白念珠菌氟康唑耐药／敏感配对菌株中的CDR1基因上游调控序列未出现任何碱基突变和缺失。结论白念珠菌氟康唑耐药与CDR1基因上游调控序列是否突变无关。 |
| 关 键 词： | 白念珠菌 氟康唑耐药 调控序列 基因突变 |
| 收稿时间： | 2005-01-12 |
| 修稿时间： | 2005-01-12 |
Preliminary study of relationship between upstream regulation sequence of Candida albicans CDR1 gene and fluconazole resistance |
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| HE Zhibin,LIAO Wanqing,YAO Zhirong,WANG Zhidong. Preliminary study of relationship between upstream regulation sequence of Candida albicans CDR1 gene and fluconazole resistance[J]. Shanghai Medical Journal, 2005, 28(8): 691-693 | |
| Authors: | HE Zhibin LIAO Wanqing YAO Zhirong WANG Zhidong |
| Abstract: | Objective To appraise the relationship between fluconazole resistance and expression of the CDR1 gene upstream regulation sequence. Methods The DNA of fluconazole-susceptible and fluconazole-resistant Candida albicans isolates were extracted, PCRs were based on the special primer of upstream regulation sequence of Candida albicans CDR1 gene, and the mutation of the sequence was compared by DNA sequencing. Results No mutation was found in the upstream regulation sequence of Candida albicans CDR1 gene. Conclusions Candida albicans fluconazole resistance is not due to mutation of the upstream regulation sequence of Candida albicans CDR1 gene. |
| Keywords: | Candida albicans Fluconazole resistance Regulation sequence Mutation |
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