山核桃树皮黄酮提取液对人肝癌Bel-7402细胞p73基因表达的影响

目的探讨山核桃树皮黄酮提取液对人肝癌Bel-7402细胞p73基因表达的影响。方法体外培养8组人肝癌Bel-7402细胞,分别采用不同浓度的山核桃树皮黄酮提取液处理后提取总RNA。采用RT-PCR法检测其p73基因剪切异构体mRNA的水平,并与正常肝细胞组进行比较。结果持续作用48 h后,黄酮提取液极低剂量组、低剂量组、中剂量组、高剂量组RT-PCR产物的目的条带灰度占总灰度的比例分别为（49.2±2.6）%、（47.5±2.2）%、（29.6±1.6）%、（13.3±1.8）%,极低、低剂量组与中、高剂量组组间比较差异均有统计学意义（P均〈0.05）。肝癌Bel-7402细胞p73基因剪切异构体mRNA水平随山核桃树皮黄酮提取液浓度增高而逐渐降低,且随剂量增加抑制效应增强。结论山核桃树皮黄酮提取液能抑制肝癌Bel-7402细胞p73基因剪切异构体的过表达,发挥抑制肿瘤细胞增殖、促进细胞凋亡的作用。

Effect of flavones extract from Juglans mandshurica Maxim stem-barks on the p73-gene expression in hepatocarcinoma cell Bel-7402

Objective To study the effect of flavones extract from Juglans mandshurica Maxim stem-barks on the p73-gene expression in hepatocarcinoma cell Bel-7402.Methods Eight groups of person liver cancer Bel-7402 cell were raised in vitro,total RNA were extracted after the cells were processing by different concentrations of Hickory bark extract flavonoids,and the mRNA of p73-gene in Bel-7402 cells were detected by RT-PCR.Results Sustained action for 48 hours,The target band grayscale amount of lower dose group,low dose group,middle dose group and high dose group were respectively（49.2±2.6）%,（47.5±2.2）%,（29.6±1.6）% and（13.3±1.8）% of total grayscale amount.Comparison among one of lower dose group and low dose group,middle dose group,high dose group were significantly different（all P0.05）.The p73-gene mRNA expression cut down gradually with the dose stepping up,and the inhibitory effect would be reinforced with the dose to raise.Conclusions The effects of flavones extact from Juglans mandshurica Maxim stem-barks inhibiting tumor cell proliferation,promoting apoptosis may be implemented by inhibiting the overexpression of the p73 gene.